Barbour H M, Davidson W
Department of Clinical Chemistry, Western General Hospital, Edinburgh, U.K.
Clin Chem. 1988 Oct;34(10):2103-5.
The "Magnesium Liquid Stable Reagent Set" from Medical Analysis Systems, Inc., is evaluated. The method, which involves Magon dye binding and bichromatic absorbance measurements, was used in a Monarch centrifugal analyzer. Results were compared with those by atomic absorption spectrometry. The calibration curve for the Magon method is linear to 2.5 mmol/L, with a 2-microL sample volume. Analytical recovery of magnesium added to human plasma ranged from 95 to 102%. The working reagent is stable for at least five days at 15 degrees C. At concentrations of 0.54 and 1.20 mmol/L, the respective CVs were 2.15 and 3.60% within batch, and 3.13 and 3.24% between batch. We analyzed 150 clinical samples for magnesium by both methods. Absorbance readings at 520/600 nm rather than 520/690 nm improved the correlation (r = 0.9777 and r = 0.9428, respectively). Calcium, albumin, phosphate, or bilirubin did not significantly interfere.
对医学分析系统公司的“镁液稳定试剂套装”进行了评估。该方法采用马贡染料结合和双色吸光度测量，在莫纳克离心分析仪中使用。将结果与原子吸收光谱法的结果进行比较。马贡法的校准曲线在2.5 mmol/L范围内呈线性，进样体积为2微升。添加到人体血浆中的镁的分析回收率在95%至102%之间。工作试剂在15℃下至少稳定五天。在浓度为0.54和1.20 mmol/L时，批内变异系数分别为2.15%和3.60%，批间变异系数分别为3.13%和3.24%。我们用这两种方法分析了150份临床样本中的镁。在520/600 nm而非520/690 nm处的吸光度读数提高了相关性（相关系数分别为r = 0.9777和r = 0.9428）。钙、白蛋白、磷酸盐或胆红素无显著干扰。
