Wang Xiu, Chen Wenyang, Jin Yiguang, Li Jianchun, Hu Yuzhen
Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, China.
Department of Pharmacy, Bengbu Medical College, Bengbu 233030, China.
J Tradit Chin Med. 2017 Jun;37(3):314-320.
To investigate the effect of Sophora flavescens alkaloid (SFA) in gel form on aerobic vaginitis (AV) and the possible mechanism underlying the effects.
AV rat models were prepared by intravaginal inoculation of Escherichia coli and Staphylococcus aureus. SFA gel and placebo gel were intravaginally administered. In vivo antibacterial effects, vaginal microenvironment, vaginal smears, pathological tissues of vaginas, and retention of gel in the vaginal cavity were investigated.
SFA gel had much higher antibacterial effect than placebo gel. SFA gel protected the vaginal mucosa from erosion of bacteria. At the same time, they inhibited the inflammatory responses, exhibiting little leukocytes and parabasal cells. Furthermore, the number of vaginal Lactobacilli remarkably increased following administration of SFA gel. However, the vaginal pH did not recover to the healthy acidic levels after treatment due to the buffering effect of gel. The gel of a fluorescent agent, Cyanine 7, showed very long retention time in the vaginal cavity, up to more than 24 h, much longer than the solutions.
The SFA gel is a promising medicine for local treatment of AV with the advantages of anti-bacteria, protection of vaginal mucosa, increase of Lactobacilli, and long retention time in the vaginal cavity.
探讨凝胶剂型的苦参生物碱（SFA）对需氧菌性阴道炎（AV）的治疗作用及其可能的作用机制。
通过阴道接种大肠杆菌和金黄色葡萄球菌制备AV大鼠模型。将SFA凝胶和安慰剂凝胶经阴道给药。研究其体内抗菌作用、阴道微环境、阴道涂片、阴道病理组织以及凝胶在阴道腔内的滞留情况。
SFA凝胶的抗菌效果远高于安慰剂凝胶。SFA凝胶可保护阴道黏膜免受细菌侵蚀。同时，它们抑制炎症反应，白细胞和基底旁细胞较少。此外，给予SFA凝胶后阴道乳酸杆菌数量显著增加。然而，由于凝胶的缓冲作用，治疗后阴道pH值未恢复到健康的酸性水平。荧光剂菁氰绿7的凝胶在阴道腔内的滞留时间非常长，长达24小时以上，远长于溶液。
SFA凝胶是一种有前景的局部治疗AV的药物，具有抗菌、保护阴道黏膜、增加乳酸杆菌以及在阴道腔内滞留时间长等优点。
