College of Life Science, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou 310053, Zhejiang, China.
Second Clinical Medical College, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou 310053, Zhejiang, China.
Molecules. 2018 Dec 29;24(1):112. doi: 10.3390/molecules24010112.
The ultrasonic-assisted extraction process and antioxidant activity of flavonoids from were investigated in this study. In order to optimize the extraction of flavonoids from , the influence of extraction time, methanol concentration, ultrasonic temperature, and solvent-to-material ratio was analyzed. Results showed that the extraction yields reached a maximum with the extraction time of 30 min, methanol concentration of 80%, temperature of 80 °C, and solvent-to-material ratio of 26 mL/g. The flavonoids were determined by HPLC, and the mean yields of trifolirhizin, formononetin, isoxanthohumol, maackiain, and kurarinone under the optimal conditions were 2.570, 0.213, 0.534, 0.797, and 3.091 mg/g, respectively. The evaluation of vitro antioxidant activity exhibited flavonoids had a strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical-scavenging ability with IC50 of 0.984 and 1.084 mg/g, respectively. These results indicate that ultrasonic-assisted extraction is an efficient approach for the selective extraction of flavonoids, and response surface methodology further optimized the extraction.
本研究旨在探讨超声辅助提取技术对 中类黄酮的提取工艺和抗氧化活性的影响。为了优化 中类黄酮的提取条件，分析了提取时间、甲醇浓度、超声温度和溶剂与原料比等因素的影响。结果表明，当提取时间为 30 min、甲醇浓度为 80%、温度为 80°C、溶剂与原料比为 26 mL/g 时，提取产率达到最大值。采用高效液相色谱法对类黄酮进行了测定，在最优条件下，三叶豆紫檀苷、芒柄花素、异黄腐醇、马栗树皮素和苦参碱的平均提取率分别为 2.570、0.213、0.534、0.797 和 3.091 mg/g。体外抗氧化活性评价结果表明，类黄酮具有较强的 1,1-二苯基-2-苦基肼（DPPH）自由基和羟基自由基清除能力，IC50 分别为 0.984 和 1.084 mg/g。这些结果表明，超声辅助提取是一种高效的选择性提取类黄酮的方法，响应面法进一步优化了提取条件。
