Jezequel M, Seta N S, Corbic M M, Feger J M, Durand G M
Laboratoire de Biochimie A, Hôpital Bichat, Paris, France.
Clin Chim Acta. 1988 Aug 15;176(1):49-57. doi: 10.1016/0009-8981(88)90173-8.
In order to test whether abnormalities in hepatocytes affect the glycoprotein carbohydrate moiety, crossed immunoaffinoelectrophoresis (CIAE) with Concanavalin A (Con A) was used to study serum alpha 1-acid glycoprotein (alpha 1-AGP) and alpha 2-HS glycoprotein (alpha 2-HS) obtained from alcoholic patients with biopsy-proven liver disease. Cirrhotic patients, placed in groups C1, C2 or C3, according to Pugh's classification, were compared to healthy donors (N) and to steatosic non-cirrhotic patients (S). Con A CIAE patterns revealed in group N three subpopulations for alpha 2-HS and four for alpha 1-AGP. Two main results emerged from this study: (1) in the alcoholic groups, the proportions of Con A-unreactive subpopulations of both glycoproteins increased. Moreover, group N could be separated from group S and group S from all the cirrhotic groups. (2) There was a good correlation between the relative amounts in Con A-unreactive subpopulations of alpha 1-AGP and alpha 2-HS. The increases observed in Con A-unreactive subpopulations are probably a general phenomenon related to alterations in glycosylation processing during liver cell damage.
为了检测肝细胞异常是否会影响糖蛋白碳水化合物部分，采用伴刀豆球蛋白A（Con A）交叉免疫亲和电泳（CIAE）技术，对经活检证实患有肝病的酒精性患者的血清α1-酸性糖蛋白（α1-AGP）和α2-HS糖蛋白（α2-HS）进行研究。根据普格分类法，将肝硬化患者分为C1、C2或C3组，并与健康供体（N）和脂肪变性非肝硬化患者（S）进行比较。Con A CIAE图谱显示，N组中α2-HS有三个亚群，α1-AGP有四个亚群。该研究得出两个主要结果：（1）在酒精性组中，两种糖蛋白的Con A不反应亚群比例均增加。此外，N组可与S组区分开，S组可与所有肝硬化组区分开。（2）α1-AGP和α2-HS的Con A不反应亚群的相对含量之间存在良好的相关性。Con A不反应亚群中观察到的增加可能是一种与肝细胞损伤期间糖基化加工改变相关的普遍现象。
