Serbource-Goguel Seta N, Durand G, Corbic M, Agneray J, Fegar J
J Hepatol. 1986;2(2):245-52. doi: 10.1016/s0168-8278(86)80083-6.
To determine whether liver damage correlates with typical changes in alpha 1-acid glycoprotein (alpha 1-AGP) carbohydrate branching, we selected patients with liver disease determined by histological liver findings. The severity of their illness was assessed by a clinical classification depending on the presence singly or together of four clinical complications (jaundice, ascites, hepatic encephalopathy and weight loss). An alpha 1-AGP crossed immunoelectrophoresis with Concanavalin A, an easy-to-perform method, revealed 3 or 4 subpopulations, the areas of which were calculated. A ratio R was determined as the most anodic peak area relative to the other ones. In our experimental conditions a ratio R value exceeding 1 correlated with the presence of one or more clinical complications. These results, evidencing fluctuations in the proportion of the carbohydrate variants of alpha 1-AGP, lead us to propose such a ratio as an index for grading liver damage. The sensitivity of this test was 86% and its specificity was 83%.
为了确定肝损伤是否与α1-酸性糖蛋白（α1-AGP）碳水化合物分支的典型变化相关，我们选择了根据肝脏组织学检查结果确诊为肝病的患者。根据四种临床并发症（黄疸、腹水、肝性脑病和体重减轻）单独或共同出现的情况，通过临床分类评估其病情严重程度。采用一种操作简便的方法，即α1-AGP与伴刀豆球蛋白A的交叉免疫电泳，可显示3或4个亚群，并计算其面积。将比率R确定为最阳极峰面积与其他峰面积的比值。在我们的实验条件下，比率R值超过1与一种或多种临床并发症的存在相关。这些结果表明α1-AGP碳水化合物变体比例存在波动，促使我们提出将该比率作为肝损伤分级指标。该检测的敏感性为86%，特异性为83%。
