Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany.
Clin Hemorheol Microcirc. 2020;74(4):405-415. doi: 10.3233/CH-190696.
The behavior of endothelial cells is remarkably influenced by the physical and biochemical signals from their surrounding microenvironments.
Here, the elasticity of fiber meshes was studied as a design parameter of substrates for endothelial cells in order to modulate angiogenesis.
Human umbilical vein endothelial cells (HUVECs) were cultured on electrospun fiber meshes made from polyetheresterurethane (PEEU), differing in their elasticity. Cell morphology, proliferation, migration and angiogenesis of endothelial cells on the degradable substrate meshes were characterized.
The aspect ratio of HUVECs cultured on the fiber meshes from PEEU materials increased with increasing stiffness of the materials. HUVECs cultured on fiber meshes with high stiffness (Young's modulus E = 4.5±0.8 MPa) presented a higher proliferation rate and significantly faster migration velocity, as well as higher tube formation capability than the cells cultured on fiber meshes with low stiffness (E = 2.6±0.8 MPa).
These results suggested that tuning the fiber meshes' elasticity might be a potential strategy for modulating the formation or regeneration of blood vessels.
内皮细胞的行为受到其周围微环境的物理和生化信号的显著影响。
本研究将纤维网的弹性作为内皮细胞基底的设计参数，以调节血管生成。
将人脐静脉内皮细胞（HUVEC）培养在由聚醚酯聚氨酯（PEEU）制成的、弹性不同的静电纺纤维网上。对可降解基底纤维网上内皮细胞的形态、增殖、迁移和血管生成进行了表征。
培养在 PEEU 材料纤维网上的 HUVEC 的长宽比随着材料硬度的增加而增加。与培养在低硬度纤维网（E=2.6±0.8 MPa）的细胞相比，培养在高硬度纤维网（E=4.5±0.8 MPa）上的 HUVEC 增殖率更高，迁移速度更快，形成管状结构的能力也更强。
这些结果表明，调整纤维网的弹性可能是调节血管形成或再生的一种潜在策略。
