Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Anal Sci. 2021 Mar 10;37(3):491-495. doi: 10.2116/analsci.20SCP10. Epub 2020 Dec 11.
Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues.
各种细胞和组织在体内通过基底膜（BMs）高度组织化，因此通过静电纺丝和层层（LbL）组装构建的有前途的人工 BM（A-BMs）最近在组织工程领域引起了广泛关注。然而，关于附着细胞在 A-BMs 上的细胞黏附、形态和迁移的控制尚未报道。在这项研究中，我们研究了 A-BMs 的厚度和粗糙度对不同组装浓度内皮细胞（ECs）功能的影响。结果表明，A-BMs 的粗糙度随纳米膜厚度的增加而逐渐增加。在具有较大粗糙度的较厚 A-BM 表面上，EC 黏附、铺展和增殖受到抑制，而通过增加接种细胞数量和延长培养时间，可补偿较厚 A-BM 表面上的内皮细胞间连接和屏障作用。我们的研究强调了 LbL 组装条件对内皮功能的影响，为组织中三维结构的 A-BMs 的设计提供了新的标准。
