莫拉司亭（重组人粒细胞-巨噬细胞集落刺激因子，rhugm-CSF，Mielogen）和来格司亭（粒细胞集落刺激因子）治疗可改善大鼠实验性结肠炎。
Treatment with Molgramostim (Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor, Rhugm-Csf, Mielogen) and Lenograstim (Granulocyte-Colony Stimulating Factor) Improves Experimental Colitis in Rats.
机构信息
Experimental, Educational and Research Center ELPEN, Athens, Greece.
European University Cyprus, School of Medicine, Nicosia, Cyprus.
出版信息
Biomed Res Int. 2019 Oct 9;2019:8298192. doi: 10.1155/2019/8298192. eCollection 2019.
BACKGROUND/AIM: Treatment with growth factors could be beneficial in both inflammatory bowel disease and experimental colitis. The aim of this study was to investigate the effect of Colony Stimulating Factor (CSF), and Recombinant Human (rHu) Granulocyte Stimulating Factor (GSF) in experimental colitis in rats.
METHODS
Experimental colitis was induced in 62 male Wistar rats, divided into 9 groups, using 2,4,6-trinitrobenzensulfonic acid (TNBS). Ten rats with colitis without treatment (control group). Euthanasia after 15 days. Ten animals with colitis without treatment (control group). Euthanasia after 30 days. Six animals with colitis. Immediate treatment with CSF. Euthanasia after 19 days. Six animals with colitis. Treatment started 7 days after the induction of colitis. Animals were kept for 19 days. Six animals with colitis. Treatment started 2 weeks after the induction of colitis. Six animals with colitis, the same as in group 3. Treatment with GSF. Six animals with colitis, the same as in group 4. Treatment with GSF.
GROUP 8: Six animals with colitis, the same as in group 5. Treatment with GSF. Six animals with colitis. Immediate treatment with prednisolone. Euthanasia after 15 days.
RESULTS
CSF and GSF administration significantly improved the histological score ( < 0.05) and reduced malondialdehyde contents ( < 0.05), compared to control groups in all animals. CSF was superior to GSF and to prednisolone.
CONCLUSION
Administration of both CSF and GSF could significantly improve the histological score and oxidative stress in experimental colitis in rats.
背景/目的：生长因子的治疗可能对炎症性肠病和实验性结肠炎都有益。本研究的目的是研究集落刺激因子（CSF）和重组人（rHu）粒细胞集落刺激因子（GSF）对大鼠实验性结肠炎的影响。
方法
使用 2,4,6-三硝基苯磺酸（TNBS）在 62 只雄性 Wistar 大鼠中诱导实验性结肠炎，将其分为 9 组。10 只结肠炎大鼠未经治疗（对照组），15 天后安乐死。10 只结肠炎大鼠未经治疗（对照组），30 天后安乐死。6 只结肠炎大鼠。立即用 CSF 治疗。19 天后安乐死。6 只结肠炎大鼠。在诱导结肠炎后 7 天开始治疗。动物饲养 19 天。6 只结肠炎大鼠。在诱导结肠炎后 2 周开始治疗。6 只结肠炎大鼠，与第 3 组相同。用 GSF 治疗。6 只结肠炎大鼠，与第 4 组相同。用 GSF 治疗。第 8 组：6 只结肠炎大鼠，与第 5 组相同。用 GSF 治疗。6 只结肠炎大鼠。立即用泼尼松龙治疗。15 天后安乐死。
结果
与所有对照组相比，CSF 和 GSF 给药均显著改善了组织学评分（<0.05）并降低了丙二醛含量（<0.05）。CSF 优于 GSF 和泼尼松龙。
结论
CSF 和 GSF 的给药均可显著改善大鼠实验性结肠炎的组织学评分和氧化应激。
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