Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Dalian Maritime University, Dalian, China.
Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; University of California, Berkeley, Berkeley, USA.
Carbohydr Res. 2019 Dec 1;486:107856. doi: 10.1016/j.carres.2019.107856. Epub 2019 Oct 24.
This study presents a quick, simple and accurate method to calculate the degree of polymerization (DP) of alginate oligosaccharides (AOS) and low molecular weight alginates from the concentration of reducing sugar determined by 3,5-dinitrosalicylic acid (DNS) assay. H NMR spectroscopy, mass spectroscopy (MS) and certified standards were used to verify the accuracy of this method, and the results showed DP calculated from DNS assay agreed with the actual DP. This method has great potential to simplify the process of measuring DP of alginate in lab and thus could be incorporated into various researches on alginates in the future. Moreover, similar method could be applied when studying the DP of other oligosaccharides.
本研究提出了一种快速、简单、准确的方法，通过 3,5-二硝基水杨酸（DNS）法测定还原糖浓度来计算海藻酸钠寡糖（AOS）和低分子量海藻酸钠的聚合度（DP）。核磁共振波谱（NMR）、质谱（MS）和标准品被用于验证该方法的准确性，结果表明，DNS 法计算的 DP 与实际 DP 相符。该方法有望简化实验室中海藻酸钠 DP 的测量过程，因此未来可能会被应用于各种海藻酸盐的研究中。此外，类似的方法也可以应用于研究其他寡糖的 DP。
