Park Jae-Ryoung, Yang Won-Tae, Kwon Yong-Sham, Kim Hyeon-Nam, Kim Kyung-Min, Kim Doh-Hoon
Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu 41566, Korea.
Department of Molecular Genetic Engineering, College of Natural Resources and Life Science, Dong-A University, Busan 49315, Korea.
Plants (Basel). 2019 Nov 4;8(11):471. doi: 10.3390/plants8110471.
The assessment of the genetic diversity within germplasm collections can be accomplished using simple sequence repeat (SSR) markers and association mapping techniques. The present study was conducted to evaluate the genetic diversity of a colored rice germplasm collection containing 376 black-purple rice samples and 172 red pericarp samples, conserved by Dong-A University. There were 600 pairs of SSR primers screened against 11 rice varieties. Sixteen informative primer pairs were selected, having high polymorphism information content (PIC) values, which were then used to assess the genetic diversity within the collection. A total of 409 polymorphic amplified fragments were obtained using the 16 SSR markers. The number of alleles per locus ranged from 11 to 47, with an average of 25.6. The average PIC value was 0.913, ranging from 0.855 to 0.964. Four hundred and nine SSR loci were used to calculate Jaccard's distance coefficients, using the unweighted pair-group method with arithmetic mean cluster analysis. These accessions were separated into several distinctive groups corresponding to their morphology. The results provided valuable information for the colored rice breeding program and showed the importance of protecting germplasm resources and the molecular markers that can be derived from them.
利用简单序列重复（SSR）标记和关联作图技术，可以完成种质资源库内遗传多样性的评估。本研究旨在评估由东亚大学保存的包含376份黑紫稻样本和172份红皮稻样本的有色稻种质资源库的遗传多样性。针对11个水稻品种筛选了600对SSR引物。选择了16对具有高多态性信息含量（PIC）值的信息性引物对，然后用于评估该资源库内的遗传多样性。使用这16个SSR标记共获得409个多态性扩增片段。每个位点的等位基因数范围为11至47，平均为25.6。平均PIC值为0.913，范围为0.855至0.964。使用非加权组平均法聚类分析，用409个SSR位点计算杰卡德距离系数。这些种质根据其形态被分为几个不同的组。研究结果为有色稻育种计划提供了有价值的信息，并表明了保护种质资源以及从中衍生出的分子标记的重要性。
