Surapaneni Malathi, Balakrishnan Divya, Mesapogu Sukumar, Krishnam Raju Addanki, Rao Yadavalli Venkateswara, Neelamraju Sarla
ICAR-National Professor Project, Indian Institute of Rice Research, Hyderabad, India.
3 Biotech. 2016 Jun;6(1):95. doi: 10.1007/s13205-016-0409-7. Epub 2016 Mar 26.
Genetic diversity among 23 rice genotypes including wild species and cultivars of indica, japonica, aus and aromatic type was investigated using 165 genomewide core set microsatellite (SSR) markers. This genotypic characterization was undertaken to know the genetic similarity among the parental lines to be used in developing a set of chromosome segment substitution lines. In all, 253 alleles were identified using 77 polymorphic SSRs, and polymorphism information content ranged from 0.31 to 0.97 with a mean of 0.79. Cluster analysis grouped the genotypes into three clusters at a genetic similarity of 0.26-0.75. Wild accessions grouped together in cluster-I, indica cultivars formed cluster-II, and aromatic, japonica and aus types came under cluster-III. Principal component analysis also showed similar results. The genotypic data was analyzed using STRUCTURE, and genotypes were grouped into four populations. RM1018 on chromosome 4, RM8009 on chromosome 7, and RM273 on chromosome 12 amplified alleles specific to wild accessions. The information obtained from core set markers would help in selecting diverse parents including wild accessions and for tracking alleles in mapping or breeding populations.
利用165个全基因组核心微卫星（SSR）标记，对包括野生稻种以及籼稻、粳稻、澳米和香稻品种在内的23个水稻基因型的遗传多样性进行了研究。进行这种基因型特征分析是为了了解用于培育一套染色体片段代换系的亲本系之间的遗传相似性。总共使用77个多态性SSR鉴定出253个等位基因，多态性信息含量范围为0.31至0.97，平均值为0.79。聚类分析在遗传相似性为0.26 - 0.75时将基因型分为三个聚类。野生种质聚在聚类I中，籼稻品种形成聚类II，香稻、粳稻和澳米类型归入聚类III。主成分分析也显示了类似结果。使用STRUCTURE对基因型数据进行分析，基因型被分为四个群体。第4染色体上的RM1018、第7染色体上的RM8009和第12染色体上的RM273扩增出了野生种质特有的等位基因。从核心标记获得的信息将有助于选择包括野生种质在内的不同亲本，并有助于在作图群体或育种群体中追踪等位基因。
