Aslanli Aysel, Stepanov Nikolay, Razheva Tatyana, Podorozhko Elena A, Lyagin Ilya, Lozinsky Vladimir I, Efremenko Elena
Faculty of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
N.M.Emanuel Institute of Biochemical Physics RAS, Moscow 119334, Russia.
Materials (Basel). 2019 Nov 4;12(21):3619. doi: 10.3390/ma12213619.
In the present work, innovative composite biomaterials possessing bactericidal properties and based on the hexahistidine-tagged organophosphorus hydrolase (His-OPH) entrapped in the poly(vinyl alcohol) cryogel (PVA-CG)/bacterial cellulose (BC) were developed. His-OPH possesses lactonase activity, with a number of N-acyl homoserine lactones being the inducers of Gram-negative bacterial resistance. The enzyme can also be combined with various antimicrobial agents (antibiotics and antimicrobial peptides) to improve the efficiency of their action. In this study, such an effect was shown for composite biomaterials when His-OPH was entrapped in PVA-CG/BC together with β-lactam antibiotic meropenem or antimicrobial peptides temporin A and indolicidin. The residual catalytic activity of immobilized His-OPH was 60% or more in all the composite samples. In addition, the presence of BC filler in the PVA-CG composite resulted in a considerable increase in the mechanical strength and heat endurance of the polymeric carrier compared to the BC-free cryogel matrix. Such enzyme-containing composites could be interesting in the biomedical field to help overcome the problem of antibiotic resistance of pathogenic microorganisms.
在本研究中，开发了一种具有杀菌特性的新型复合生物材料，该材料基于包裹在聚乙烯醇冷冻凝胶（PVA-CG）/细菌纤维素（BC）中的六组氨酸标签有机磷水解酶（His-OPH）。His-OPH具有内酯酶活性，许多N-酰基高丝氨酸内酯是革兰氏阴性菌耐药性的诱导剂。该酶还可与多种抗菌剂（抗生素和抗菌肽）结合，以提高其作用效率。在本研究中，当His-OPH与β-内酰胺抗生素美罗培南或抗菌肽天蚕素A和吲哚杀菌素一起包裹在PVA-CG/BC中时，复合生物材料表现出了这样的效果。在所有复合样品中，固定化His-OPH的残余催化活性均达到60%或更高。此外，与不含BC的冷冻凝胶基质相比，PVA-CG复合材料中BC填料的存在导致聚合物载体的机械强度和耐热性显著提高。这种含酶复合材料在生物医学领域可能会引起人们的兴趣，有助于克服病原微生物的抗生素耐药性问题。
