Yap K L, Ada G L
Scand J Immunol. 1979;10(4):325-32. doi: 10.1111/j.1365-3083.1979.tb01358.x.
A study has been made of the effect of humoral antibody on the generation of specific cytotoxic T cells (Tc) in the spleen or lungs after intravenous injection or intranasal inoculation of infectious influenza virus. Antibody injected before or at the same time as virus inhibited completely the generation of Tc in the spleen. If injected 1 h after virus, the inhibition was reduced by 50%, and little inhibition occurred if antibody was injected 6 h after virus. This suggested that antibody failed to influence Tc generation once infection of stimulating cells had occurred. Antibody injected intravenously 24 h after intranasal inoculation of virus into normal mice did not affect the level of cytotoxic activity present in the lungs, and trace amounts only (less than 1 log10 EID50) could be recovered from the lungs at 6 days. As there is a high titre (greater than 6 log10 EID50) of infectious virus in the lungs of mice 24 h after infection, this represents a very efficient control mechanism. The same protocol carried out with athymic mice gave only a partial clearance (c. 3 log10 EID50) of virus in the lungs. It was suggested that a major role of humoral antibody was to limit infection by the virus, and in this respect it complemented the action of Tc.
一项研究探讨了体液抗体对静脉注射或鼻内接种传染性流感病毒后脾脏或肺部特异性细胞毒性T细胞（Tc）产生的影响。在病毒注射前或同时注射抗体可完全抑制脾脏中Tc的产生。如果在病毒注射后1小时注射，抑制作用降低50%，而在病毒注射后6小时注射抗体则几乎没有抑制作用。这表明一旦刺激细胞发生感染，抗体就无法影响Tc的产生。在正常小鼠鼻内接种病毒24小时后静脉注射抗体，并不影响肺部存在的细胞毒性活性水平，且在6天时仅能从肺部回收微量（小于1 log10 EID50）的抗体。由于感染后24小时小鼠肺部存在高滴度（大于6 log10 EID50）的传染性病毒，这代表了一种非常有效的控制机制。对无胸腺小鼠进行相同方案的实验，肺部病毒仅得到部分清除（约3 log10 EID50）。研究表明，体液抗体的主要作用是限制病毒感染，在这方面它补充了Tc的作用。
