Lopez Genghis H, Wilson Brett, Turner Robyn M, Millard Glenda M, Fraser Nicole S, Roots Naomi M, Liew Yew-Wah, Hyland Catherine A, Flower Robert L
Research and Development Laboratory, Clinical Services and Research Division, Australian Red Cross Blood Service, Kelvin Grove, Queensland, Australia.
Red Cell Reference Laboratory, Clinical Services and Research Division, Australian Red Cross Blood Service, Kelvin Grove, Queensland, Australia.
Transfus Med Hemother. 2020 Jul;47(4):279-286. doi: 10.1159/000504026. Epub 2019 Nov 14.
MNS blood group system genes and share a high degree of sequence homology and gene structure. Homologous exchanges between and form hybrid genes encoding hybrid glycophorins GP(A-B-A) and GP(B-A-B). Over 20 hybrid glycophorins have been characterised. Each has a distinct phenotype defined by the profile of antigens expressed including Mi. Seven hybrid glycophorins carry Mi and have been reported in Caucasian and Asian population groups. In Australia, the population is diverse; however, the prevalence of hybrid glycophorins in the population has never been determined. The aims of this study were to determine the frequency of Mi and to classify Mi-positive hybrid glycophorins in an Australian blood donor population.
Blood samples from 5,098 Australian blood donors were randomly selected and screened for Mi using anti-Mi monoclonal antibody (CBC-172) by standard haemagglutination technique. Mi-positive red blood cells (RBCs) were further characterised using a panel of phenotyping reagents. Genotyping by high-resolution melting analysis and DNA sequencing were used to confirm serology.
RBCs from 11/5,098 samples were Mi-positive, representing a frequency of 0.22%. Serological and molecular typing identified four types of Mi-positive hybrid glycophorins: GP.Hut ( = 2), GP.Vw ( = 3), GP.Mur ( = 5), and 1 GP.Bun ( = 1). GP.Mur was the most common.
This is the first comprehensive study on the frequency of Mi and types of hybrid glycophorins present in an Australian blood donor population. The demographics of Australia are diverse and ever-changing. Knowing the blood group profile in a population is essential to manage transfusion needs.
MNS血型系统基因 与 具有高度的序列同源性和基因结构。 与 之间的同源交换形成了编码杂合糖蛋白GP(A - B - A)和GP(B - A - B)的杂交基因。已鉴定出20多种杂合糖蛋白。每种都有由所表达抗原谱定义的独特表型，包括Mi。七种杂合糖蛋白携带Mi，已在白种人和亚洲人群体中报道。在澳大利亚，人口多样化；然而，杂合糖蛋白在该人群中的流行率从未确定。本研究的目的是确定澳大利亚献血人群中Mi的频率，并对Mi阳性杂合糖蛋白进行分类。
随机选取5098名澳大利亚献血者的血样，采用标准血凝技术，使用抗Mi单克隆抗体（CBC - 172）筛选Mi。使用一组表型试剂对Mi阳性红细胞（RBC）进行进一步鉴定。通过高分辨率熔解分析和DNA测序进行基因分型以确认血清学结果。
5098份样本中有11份的RBC为Mi阳性，频率为0.22%。血清学和分子分型鉴定出四种类型的Mi阳性杂合糖蛋白：GP.Hut（ = 2）、GP.Vw（ = 3）、GP.Mur（ = 5）和1份GP.Bun（ = 1）。GP.Mur最为常见。
这是首次对澳大利亚献血人群中Mi的频率和杂合糖蛋白类型进行的全面研究。澳大利亚的人口结构多样且不断变化。了解人群中的血型谱对于满足输血需求至关重要。
