Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Traditional Chinese Medicine Sciences, Chengdu 610041, PR China.
Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Traditional Chinese Medicine Sciences, Chengdu 610041, PR China; Sichuan Agricultural University, Chengdu 611134, PR China.
Fitoterapia. 2020 Jan;140:104412. doi: 10.1016/j.fitote.2019.104412. Epub 2019 Nov 5.
Aconitum carmichaelii has been used in traditional Chinese medicine for treating various diseases for several thousand years. Based on the biosynthetic pathway of some alkaloids such as C19-diterpenoid alkaloids and obvious differences in alkaloid content between leaves of two A. carmichaelii varieties has been reported, we performed leaves transcriptome analysis of two A. carmichaelii varieties. Besides we characterized the biosynthetic pathway of salsolinol. A total of 56 million raw reads (8.28 G) and 55 million clean reads (8.24 G) were obtained from two varieties (Z175 and R184) leaves transcriptome, respectively, and 176,793 unigenes were annotated. 281 and 843 unigenes are involved in the salsolinol biosynthetic pathway and the formation of C19-diterpenoid alkaloids respectively. And including 34 and 24 unigenes are the differentially expressed genes (DEGs) in the biosynthesis pathway for C19-diterpenoid alkaloids and salsolinol between Z175 and R184 respectively, which were target genes to explore differences in C19-diterpenoid alkaloid and salsolinol biosynthesis in Z175 and R184. Thus genes involved in alkaloid biosynthesis and accumulation differ between varieties leaves. The mechanisms underlying the differences and their relevance require further exploration. The results expand our knowledge of alkaloids biosynthesis in A. carmichaelii leaves, and provide a theoretical basis for analysis differences in alkaloids biosynthesis patterns in different varieties.
乌头属植物已在传统中医中使用了数千年，用于治疗各种疾病。基于一些生物碱（如 C19-二萜生物碱）的生物合成途径，以及两种乌头属植物品种叶片中生物碱含量的明显差异，我们对两种乌头属植物品种的叶片转录组进行了分析。此外，我们还对桑辛醇的生物合成途径进行了表征。从两个品种（Z175 和 R184）的叶片转录组中分别获得了 5600 万条原始读数（8.28 G）和 5500 万条清洁读数（8.24 G），并对 176793 个 unigenes 进行了注释。分别有 281 个和 843 个 unigenes参与了桑辛醇生物合成途径和 C19-二萜生物碱的形成。包括 34 个和 24 个 unigenes分别是 C19-二萜生物碱和桑辛醇生物合成途径中 Z175 和 R184 之间的差异表达基因（DEGs），这些差异表达基因是探索 Z175 和 R184 中 C19-二萜生物碱和桑辛醇生物合成差异的靶基因。因此，品种叶片中生物碱生物合成和积累所涉及的基因存在差异。需要进一步探索差异的机制及其相关性。研究结果扩展了我们对乌头属植物叶片生物碱生物合成的认识，为分析不同品种生物碱生物合成模式的差异提供了理论依据。
