Huang Qinghua, Fei Xianming, Li Shengbing, Xu Chan, Tu Chunping, Jiang Lei, Wo Mingyi
Department of Endocrinology, Zhejiang Provincial People's Hospital and People's Hospital of Hangzhou Medical College, Hangzhou 310014, China.
Center for Laboratory Medicine, Zhejiang Provincial People's Hospital and People's Hospital of Hangzhou Medical College, Hangzhou 310014, China.
Ann Transl Med. 2019 Sep;7(18):483. doi: 10.21037/atm.2019.08.75.
Cyclooxygenase-2 (COX-2) plays an important role in the monocyte-platelet aggregate (MPA)-medicated inflammatory response and possible coronary artery disease (CAD). This study aimed to assess the predicting significance of COX-2 expression in peripheral blood monocyte for CAD.
A total of 66 patients with CAD including stable angina (SA) and unstable angina (UA) were enrolled. The inflammatory indexes including white blood cell (WBC) count, high-sensitive C reactive protein (hs-CRP), serum monocyte chemoattractant protein-1 (MCP-1) and MPA levels were measured. The western-blotting assay and reverse transcription-polymerase chain reaction (RT-PCR) analysis were used to detect the COX-2 expression in peripheral blood monocytes. Furthermore, the correlation between COX-2 expression and MPA levels, and the association of COX-2 expression with CAD risk were assessed.
The UA patients demonstrated higher levels of inflammatory indexes than the SA patients (P<0.001). Simultaneously, higher MPA levels and enhanced COX-2 expression were observed in the UA patients (P<0.01). The patients with enhanced COX-2 expression exhibited higher MPA than those without (P<0.01), and patients with increased MPA also demonstrated enhanced COX-2 expression (P<0.001). Moreover, the levels of COX-2 protein expression was positively related to the MPA formation rates (R=0.4933, P<0.01), and enhanced COX-2 expression was independently associated with CAD risk [odds ratio (OR): 6.322, 95% confidence interval (CI): 4.544-8.978 ].
The COX-2 expression of peripheral blood monocytes can be used as an independent predictor for CAD.
环氧化酶 -2（COX-2）在单核细胞 - 血小板聚集体（MPA）介导的炎症反应及可能的冠状动脉疾病（CAD）中起重要作用。本研究旨在评估外周血单核细胞中COX-2表达对CAD的预测意义。
共纳入66例CAD患者，包括稳定型心绞痛（SA）和不稳定型心绞痛（UA）患者。检测炎症指标，包括白细胞（WBC）计数、高敏C反应蛋白（hs-CRP）、血清单核细胞趋化蛋白-1（MCP-1）和MPA水平。采用蛋白质免疫印迹法和逆转录 - 聚合酶链反应（RT-PCR）分析检测外周血单核细胞中COX-2的表达。此外，评估COX-2表达与MPA水平之间的相关性，以及COX-2表达与CAD风险的关联。
UA患者的炎症指标水平高于SA患者（P<0.001）。同时，UA患者中观察到较高的MPA水平和增强的COX-2表达（P<0.01）。COX-2表达增强的患者比未增强的患者表现出更高的MPA水平（P<0.01），MPA升高的患者也表现出COX-2表达增强（P<0.001）。此外，COX-2蛋白表达水平与MPA形成率呈正相关（R = 0.4933，P<0.01），COX-2表达增强与CAD风险独立相关[比值比（OR）：6.322，95%置信区间（CI）：4.544 - 8.978]。
外周血单核细胞的COX-2表达可作为CAD的独立预测指标。
