Eutypellacytosporins A-D，北极真菌 sp. D-1 来源的混合萜类化合物

Eutypellacytosporins A-D, Meroterpenoids from the Arctic Fungus sp. D-1.

机构信息

Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences , Second Military Medical University , Shanghai 200433 , People's Republic of China.

School of Pharmacy , Second Military Medical University , Shanghai 200433 , People's Republic of China.

出版信息

J Nat Prod. 2019 Nov 22;82(11):3089-3095. doi: 10.1021/acs.jnatprod.9b00700. Epub 2019 Nov 8.

DOI:10.1021/acs.jnatprod.9b00700

PMID:31702148

Abstract

The Arctic fungus sp. D-1, previously found to produce a variety of cytotoxic cyclopropyl-fused and cyclobutyl-fused pimarane diterpenoids when grown in the defined medium, was induced to produce unusual metabolites by growing on solid rice medium. A chemical investigation on the rice medium extract led to the isolation of four new meroterpenoids, eutypellacytosporins A-D (-), along with the known biogenetically related compound cytosporin D (). The structures of the new compounds were elucidated by their detailed spectroscopic analysis and modified Mosher's method. Compounds - may be formed by the 12,32-ester linkage of two moieties, cytosporin D () and decipienolide A or B. All isolated compounds, except , showed weak cytotoxicity against DU145, SW1990, Huh7, and PANC-1 cell lines with IC values ranging from 4.9 to 17.1 μM.

摘要

先前在限定培养基中培养时，真菌 sp. D-1 产生了多种细胞毒性的环丙基稠合和环丁基稠合的松香二萜，当在固体大米培养基中生长时，被诱导产生了不寻常的代谢物。对大米培养基提取物的化学研究导致分离出四个新的混合萜类化合物，eutypellacytosporins A-D (-)，以及已知的生物合成相关化合物 cytosporin D ()。新化合物的结构通过其详细的光谱分析和改进的 Mosher 法阐明。化合物 - 可能是由两个部分，cytosporin D ()和 decipienolide A 或 B 的 12,32-酯键形成的。除了外，所有分离出的化合物对 DU145、SW1990、Huh7 和 PANC-1 细胞系均显示出较弱的细胞毒性，IC 值范围为 4.9 至 17.1 μM。