Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, and Marine Biopharmaceutical Institute, Second Military Medical University, Shanghai 200433, China.
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, CA 92093, USA.
Mar Drugs. 2018 Aug 16;16(8):284. doi: 10.3390/md16080284.
Three new pimarane diterpenes, eutypellenoids A⁻C (⁻), together with a known compound, eutypenoid C (), were isolated from the culture extract of sp. D-1 derived from the Arctic region. Compounds ⁻ possessed an uncommon tetrahydrofuran-fused pimarane diterpene skeleton. The structures of all compounds were determined by detailed spectroscopic analysis, electronic circular dichroism (ECD) analysis, as well as a comparison with the literature data. Antibacterial, antifungal, and cytotoxic activities of these compounds were evaluated. Compound displayed antibacterial activity against and with MIC values of 8 and 8 μg/mL, respectively. Additionally, compound showed antifungal activity against , , , and with MIC values of 8, 8, 16, and 32 μg/mL, respectively. Furthermore, compound exhibited moderate cytotoxic activity against HCT-116 cell line with IC value of 3.7 μM.
从北极地区的 sp. D-1 衍生的培养提取物中分离得到了三种新的松香烷二萜，分别为 eutypellenoids A⁻C（⁻），以及一种已知化合物 eutypenoid C（）。化合物 ⁻ 具有不常见的四氢呋喃稠合松香烷二萜骨架。通过详细的光谱分析、电子圆二色性（ECD）分析以及与文献数据的比较，确定了所有化合物的结构。评估了这些化合物的抗菌、抗真菌和细胞毒性活性。化合物 对 和 显示出抗菌活性，MIC 值分别为 8 和 8 μg/mL。此外，化合物 对 、 、 、 和 显示出抗真菌活性，MIC 值分别为 8、8、16、32 μg/mL。此外，化合物 对 HCT-116 细胞系表现出中等的细胞毒性活性，IC 值为 3.7 μM。
