Department of Chemistry , Imperial College London , Molecular Sciences Research Hub, White City Campus, Wood Lane , London W12 0BZ , U.K.
Langmuir. 2019 Dec 17;35(50):16521-16527. doi: 10.1021/acs.langmuir.9b02336. Epub 2019 Dec 4.
Dispersions of nonlamellar lipid membrane assemblies are gaining increasing interest for drug delivery and protein therapeutic application. A key bottleneck has been the lack of rational design rules for these systems linking different lipid species and conditions to defined lattice parameters and structures. We have developed robust methods to form cubosomes (nanoparticles with porous internal structures) with water channel diameters of up to 171 Å, which are over 4 times larger than archetypal cubosome structures. The water channel diameter can be tuned via the incorporation of cholesterol and the charged lipid DOPA, DOPG, or DOPS. We have found that large molecules can be incorporated into the porous cubosome structure and that these molecules can interact with the internal cubosome membrane. This offers huge potential for accessible encapsulation and protection of biomolecules and development of confined interfacial reaction environments.
非层状脂质膜组装体的分散体因其在药物输送和蛋白质治疗应用方面的潜力而受到越来越多的关注。一个关键的瓶颈是缺乏将不同的脂质种类和条件与特定的晶格参数和结构联系起来的合理设计规则。我们已经开发了形成具有高达 171Å 的水通道直径的立方纳米囊泡（具有多孔内部结构的纳米颗粒）的稳健方法，这比典型的立方纳米囊泡结构大 4 倍以上。水通道直径可以通过胆固醇和带电荷的脂质 DOPA、DOPG 或 DOPS 的掺入来调节。我们发现大分子可以被掺入到多孔的立方纳米囊泡结构中，并且这些分子可以与内部的立方纳米囊泡膜相互作用。这为可及的封装和保护生物分子以及开发受限的界面反应环境提供了巨大的潜力。
