Laboratory of Preclinical Research of Natural Products, Postgraduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, Paraná, Brazil.
Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.
J Med Food. 2020 Jun;23(6):676-684. doi: 10.1089/jmf.2019.0165. Epub 2019 Nov 8.
Cardiovascular disease (CVD) is the leading cause of death worldwide and among its modifiable risk factors are dyslipidemia, diabetes, and smoking. Experimental models evaluated this risk factors singly, however, there is a lack of models that agglomerate these risk factors, resembling real patients and elucidating the pathophysiology of CVD. Moreover, few studies have investigated the cardioprotective effects of , a species with lipid-lowering effects. In this study, ethanol-soluble fraction of was characterized by liquid chromatography-mass spectrometry. Diabetes was induced by streptozotocin in Wistar rats that also received 0.5% cholesterol-enriched chow and were exposed to the smoke of nine cigarettes, 5 days/week, for 4 weeks. During the last 2 weeks, the animals were treated with vehicle (C), , or simvastatin plus insulin. At the end, cholesterol, triglyceride, urea, and creatinine levels; blood pressure (BP); heart rate (HR); abdominal aortic morphometry; vascular reactivity; renal and cardiac oxidative status; and histopathological changes were evaluated. The agglomerate of risk factors promoted alterations contrary to those described in the literature for the isolated risk factors. The C group exhibited oxidative stress, increase in biochemical parameters, and thickening of the wall of the abdominal aorta. HR, systolic, diastolic, and mean BP decreased, and vascular reactivity was altered. Cardiac and renal histopathological changes were observed. Treatment with reversed these changes and this effect may be partially attributable to lipid-lowering action and to the inhibition of free radical generation. has cardioprotective effects in this model, with no toxicity.
心血管疾病 (CVD) 是全球范围内的主要死亡原因，其可改变的危险因素包括血脂异常、糖尿病和吸烟。实验模型单独评估了这些危险因素，但缺乏聚集这些危险因素的模型，无法模拟真实患者并阐明 CVD 的病理生理学。此外，很少有研究调查 具有降脂作用的 对心脏的保护作用。在这项研究中，用液相色谱-质谱法对 的乙醇可溶部分进行了表征。通过链脲佐菌素诱导 Wistar 大鼠产生糖尿病，同时给予富含 0.5%胆固醇的饲料，并让它们每周 5 天、每天 5 支香烟暴露于烟雾中 4 周。在最后 2 周，动物用载体 (C)、 或辛伐他汀加胰岛素治疗。最后，评估了胆固醇、甘油三酯、尿素和肌酐水平、血压 (BP)、心率 (HR)、腹主动脉形态计量学、血管反应性、肾和心脏氧化状态以及组织病理学变化。危险因素的聚集导致与单独危险因素描述的相反的变化。C 组表现出氧化应激、生化参数增加和腹主动脉壁增厚。HR、收缩压、舒张压和平均 BP 降低，血管反应性改变。观察到心脏和肾脏的组织病理学变化。用 治疗逆转了这些变化，这种效果可能部分归因于降脂作用和自由基生成的抑制。 在该模型中具有心脏保护作用，且无毒性。
