Wang Jiefei, Wang Zhongjie, Zhong Yong, Zou Yan, Wang Chong, Wu Haigang, Lee Albert, Yang Weitao, Wang Xiao, Liu Yanjie, Zhang Dongya, Yan Jiliang, Hao Mingcong, Zheng Meng, Chung Roger, Bai Feng, Shi Bingyang
International Joint Center for Biomedical Innovation, School of Life Sciences Henan University, Kaifeng, 475004, China.
Key Laboratory for Special Functional Materials of Ministry of Education, National & Local Joint Engineering Research Center for High-efficiency Display and Lighting Technology, School of Materials Science and Engineering, Collaborative Innovation Center of Nano Functional Materials and Applications, Henan University, Kaifeng, 475004, China.
Biomaterials. 2020 Jan;229:119576. doi: 10.1016/j.biomaterials.2019.119576. Epub 2019 Oct 24.
Dual-modal imaging guided photodynamic therapy (PDT) of multifunctional nanocomposites holds great promise for precision tumor theranostics. However, poor heterogeneous interfacial compatibility between functional components, low hydrophilicity and complicated preparation of nanocomposites remain major obstacles for further bioapplication. Herein, a facile central metal-derived co-assembly strategy is developed to effectively integrate gadolinium porphyrin (GdTPP) contrast agent and Zinc porphyrin (ZnTPP) photosensitizer into a homogeneous GdTPP/ZnTPP nanocomposites (GZNs). GZNs possesses the following advantages: (1) Greatly improved interfacial compatibility facilitated by incorporating two metalporphyrins with same group (phenyl-) and different central metal atoms (Zn and Gd) leading to higher yield (4.7-5 fold) than either monocomponent nanoparticles. (2) Poor dispersity of GdTPP nanoparticles is greatly improved after integrating with ZnTPP blocks. (3) The GZNs inherit excellent fluorescence imaging, high relaxation rate (8.18 mM s) and singlet oxygen production from two raw metalporphyrins. After camouflaging with homotypic cancer cell membrane for immunologic escape, the HeLa membrane coated GZNs (mGZNs) show enhanced in vivo MR/FL imaging guided anti-tumor targeting efficiency of 80.6% for HeLa cells. Our new strategy using central metal-derived co-assembly of homogeneous building blocks greatly improves interfacial compatibility to achieve combined functions for visualized cancer theranostics.
多功能纳米复合材料的双模态成像引导光动力疗法（PDT）在精准肿瘤诊疗方面具有巨大潜力。然而，功能组分之间不良的异质界面相容性、低亲水性以及纳米复合材料复杂的制备过程仍然是其进一步生物应用的主要障碍。在此，我们开发了一种简便的中心金属衍生共组装策略，以有效地将钆卟啉（GdTPP）造影剂和锌卟啉（ZnTPP）光敏剂整合到均匀的GdTPP/ZnTPP纳米复合材料（GZNs）中。GZNs具有以下优点：（1）通过引入具有相同基团（苯基-）和不同中心金属原子（锌和钆）的两种金属卟啉，极大地改善了界面相容性，使得产率比单组分纳米颗粒提高了4.7 - 5倍。（2）与ZnTPP嵌段整合后，GdTPP纳米颗粒的差分散性得到了极大改善。（3）GZNs继承了两种原始金属卟啉的优异荧光成像、高弛豫率（8.18 mM s）和单线态氧产生能力。在用同型癌细胞膜进行伪装以实现免疫逃逸后，包被HeLa细胞膜的GZNs（mGZNs）对HeLa细胞的体内MR/FL成像引导抗肿瘤靶向效率提高到了80.6%。我们利用中心金属衍生共组装均一构建块的新策略极大地改善了界面相容性，以实现可视化癌症诊疗的联合功能。
