组织和血清中黑素转铁蛋白水平可作为胃癌的预后标志物。
Level of Melanotransferrin in Tissue and Sera Serves as a Prognostic Marker of Gastric Cancer.
机构信息
Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan
出版信息
Anticancer Res. 2019 Nov;39(11):6125-6133. doi: 10.21873/anticanres.13820.
AIM
The aim of the study was to identify novel biomarkers that are vital for improving management of patients with gastric cancer (GC).
MATERIALS AND METHODS
An RNA-sequencing analysis was conducted using gastric tissue from patients with metastatic GC. In vitro cell functions were evaluated by siRNA-mediated knockdown assays. A total of 230 pairs of gastric tissue were subjected to expression analysis of mRNA and protein in situ. The serum levels of the candidate biomarker were determined by ELISA.
RESULTS
MELTF was identified as a candidate biomarker. Inhibition of MELTF expression suppressed the invasion ability of GC cells. Increased tissue MELTF mRNA expression was associated with shorter survival. Furthermore, staining intensity of tissue MELTF protein was linked to recurrence rates. Serum MELTF levels gradually were increased from healthy controls to advanced GC. Patients with high serum MELTF levels had poor prognosis.
CONCLUSION
Both tissue and serum MELTF levels may serve as biomarkers of GC progression.
目的
本研究旨在确定对改善胃癌（GC）患者管理至关重要的新型生物标志物。
材料和方法
使用转移性 GC 患者的胃组织进行 RNA 测序分析。通过 siRNA 介导的敲低实验评估体外细胞功能。对 230 对胃组织进行了 mRNA 和蛋白原位表达分析。通过 ELISA 测定候选生物标志物的血清水平。
结果
MELTF 被鉴定为候选生物标志物。抑制 MELTF 表达可抑制 GC 细胞的侵袭能力。组织中 MELTF mRNA 表达增加与生存时间缩短相关。此外，组织 MELTF 蛋白染色强度与复发率相关。血清 MELTF 水平从健康对照逐渐升高到晚期 GC。血清 MELTF 水平高的患者预后不良。
结论
组织和血清 MELTF 水平均可作为 GC 进展的生物标志物。
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