Robin E D, Wong R
Department of Medicine, Stanford University School of Medicine, California 94305.
J Cell Physiol. 1988 Sep;136(3):507-13. doi: 10.1002/jcp.1041360316.
A new biochemical method for estimating the virtual number of mitochondria (mt) per cell was developed and used together with a plasmid probe to measure mt DNA/mitochondrion and mt DNA/cell. These methods were used in five cell types from four mammalian species. Mt DNA/mitochondrion was essentially constant in all cell types (mean 2.6 +/- 0.30 SE mitochondrial DNA molecules/mt). Mt DNA molecules/cell encompassed an eight-fold range between various cell types (low 220 +/- 6.2; high 1,720 +/- 162 mt DNA molecules/cell). Virtual mt number/cell ranged from 83 +/- 17 to 677 +/- 80 (SE) mt/cell in various cell types. All five mammalian virtual mitochondria contained the same genomic mass. The number of virtual mitochondria per cell and amount of mt DNA per cell appear to be closely regulated within a given cell type but differ widely from cell type to cell type.
一种用于估算每个细胞中线粒体（mt）虚拟数量的新生化方法被开发出来，并与质粒探针一起用于测量线粒体DNA/线粒体和线粒体DNA/细胞。这些方法被应用于来自四种哺乳动物的五种细胞类型。线粒体DNA/线粒体在所有细胞类型中基本恒定（平均2.6 +/- 0.30标准误线粒体DNA分子/线粒体）。线粒体DNA分子/细胞在不同细胞类型之间涵盖了八倍的范围（低220 +/- 6.2；高1720 +/- 162线粒体DNA分子/细胞）。不同细胞类型中每个细胞的虚拟线粒体数量范围为83 +/- 17至677 +/- 80（标准误）线粒体/细胞。所有五种哺乳动物的虚拟线粒体都包含相同的基因组质量。每个细胞中的虚拟线粒体数量和每个细胞中的线粒体DNA数量在给定的细胞类型内似乎受到密切调节，但在不同细胞类型之间差异很大。
