Hasegawa Y, Kunihara M, Maruyama Y
J Chromatogr. 1982 Apr 30;239:335-42. doi: 10.1016/s0021-9673(00)81992-5.
Blood levels of choline (Ch) and acetylcholine (ACh) have previously not been confirmed because of the difficulty in measuring these compounds. In this paper a recently developed method for the assay of Ch and ACh, which employs a chemical ionization (isobutane) quadrupole mass spectrometer (JEOL QH 100) equipped with a new type of pyrolyzer is reported. The correlation between the mass fragmentogram peak ratios and the amounts of compounds was good from 0.5 to 4 pmol. The assay limit for quantitation was ca. 0.3 pmol for Ch and ACh. The values for Ch from rat whole blood, serum and red blood cells were 10.88 +/- 1.46, 6.72 +/- 1.02 and 4.56 +/- 0.53 nmol/ml, respectively, and for ACh the respective values were 3.56 +/- 0.86, 1.69 +/- 0.16 and 1.87 +/- 0.83 nmol/ml. In human whole blood the levels of Ch and ACh were 22.55 +/- 3.97 and 3.23 +/- 0.23, respectively.
由于测量这些化合物存在困难，胆碱（Ch）和乙酰胆碱（ACh）的血液水平此前尚未得到证实。本文报道了一种最近开发的Ch和ACh测定方法，该方法采用配备新型热解器的化学电离（异丁烷）四极杆质谱仪（JEOL QH 100）。质量碎片图峰比与化合物含量之间在0.5至4皮摩尔范围内具有良好的相关性。Ch和ACh的定量测定限约为0.3皮摩尔。大鼠全血、血清和红细胞中Ch的值分别为10.88±1.46、6.72±1.02和4.56±0.53纳摩尔/毫升，ACh的相应值分别为3.56±0.86、1.69±0.16和1.87±0.83纳摩尔/毫升。在人类全血中，Ch和ACh的水平分别为22.55±3.97和3.23±0.23。
