Song Jianyang, Li Minghui, Wang Chunyan, Fan Yujie, Li Yuan, Wang Yongkun, Zhang Wenxiao, Li Haisong, Wang Hongyu
Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, Nanyang Institute of Technology, Nanyang, China.
School of Civil Engineering, Nanyang Institute of Technology, Nanyang, China.
Front Microbiol. 2023 Feb 8;14:1139650. doi: 10.3389/fmicb.2023.1139650. eCollection 2023.
In this work, polyvinyl alcohol (PVA) and sodium alginate (SA) were used as entrapped carriers and stem biochar (ABC) was used as an absorption carrier to immobilize aerobic denitrifying bacteria screened from landfill leachate, thus a new carbon-based functional microbial material (PVA/SA/ABC@BS) was successfully prepared.
The structure and characteristics of the new material were revealed by using a scanning electron microscope and Fourier transform infrared spectroscopy, and the performance of the material for treating landfill leachate under different working conditions was studied.
ABC had abundant pore structures and that the surface contained many oxygen-containing functional groups, carboxyl groups, and amide groups, etc. and it had good absorbing performance and strong acid and alkali buffering capacity, which was beneficial to the adhesion and proliferation of microorganisms. After adding ABC as a composite carrier, the damage rate of immobilized particles was decreased by 1.2%, and the acid stability, alkaline stability, and mass transfer performance were increased by 9.00, 7.00, and 56%, respectively. When the dosage of PVA/SA/ABC@BS was 0.017g/ml, the removal rates of nitrate nitrogen (NO -N) and ammonia nitrogen (NH -N) were the highest, which were 98.7 and 59.4%, respectively. When the pH values were 11, 7, 1, and 9, the removal rates of chemical oxygen demand (COD), NO -N, nitrite nitrogen (NO -N) and NH -N reached the maximum values, which were 14.39, 98.38, 75.87, and 79.31%, respectively. After PVA/SA/ABC@BS was reused in 5 batches, the removal rates of NO -N all reached 95.50%.
PVA, SA and ABC have excellent reusability for immobilization of microorganisms and degradation of nitrate nitrogen. This study can provide some guidance for the great application potential of immobilized gel spheres in the treatment of high concentration organic wastewater.
本研究以聚乙烯醇（PVA）和海藻酸钠（SA）作为包埋载体，以秸秆生物炭（ABC）作为吸附载体，固定化从垃圾渗滤液中筛选出的好氧反硝化细菌，成功制备了一种新型碳基功能微生物材料（PVA/SA/ABC@BS）。
利用扫描电子显微镜和傅里叶变换红外光谱对新材料的结构和特性进行表征，并研究该材料在不同工况下处理垃圾渗滤液的性能。
ABC具有丰富的孔隙结构，表面含有许多含氧官能团、羧基和酰胺基等，具有良好的吸附性能和较强的酸碱缓冲能力，有利于微生物的附着和增殖。添加ABC作为复合载体后，固定化颗粒的破损率降低了1.2%，酸稳定性、碱稳定性和传质性能分别提高了9.00%、7.00%和56%。当PVA/SA/ABC@BS投加量为0.017g/ml时，硝酸盐氮（NO₃-N）和氨氮（NH₃-N）的去除率最高，分别为98.7%和59.4%。当pH值分别为11、7、1和9时，化学需氧量（COD）、NO₃-N、亚硝酸盐氮（NO₂-N）和NH₃-N的去除率达到最大值，分别为14.39%、98.38%、75.87%和79.31%。PVA/SA/ABC@BS重复使用5批次后，NO₃-N的去除率均达到95.50%。
PVA、SA和ABC对微生物固定化及硝酸盐氮降解具有优良的重复使用性。本研究可为固定化凝胶球在高浓度有机废水处理中的巨大应用潜力提供一定指导。
