Di Benedetto Nilde Antonella, Campaniello Daniela, Bevilacqua Antonio, Cataldi Mariagrazia Pia, Sinigaglia Milena, Flagella Zina, Corbo Maria Rosaria
Department of the Science of Agriculture, Food and Environment (SAFE), University of Foggia, 71122 Foggia, Italy.
Microorganisms. 2019 Nov 8;7(11):541. doi: 10.3390/microorganisms7110541.
The main goal of this paper was to select promising microorganisms which could potentially act as plant-growth-promoting bacteria (PGPB) for durum wheat of Foggia County. At this scope, a new statistical framework, based on multivariate analyses and the evaluation of the statistical distribution of each trait, was used. Four hundred and seventy-four isolates were isolated from the rhizosphere of durum wheat in Foggia County and preliminarily screened as a function of four target indices (ammonium production, siderophores production, P-solubilization, and nitrification). After this step, the number of strains was reduced and the remaining isolates were tested through a quantitative approach, to assess the production of IAA (indole acetic acid), P-mineralization, and nitrification. In this second step, the cut-off was based on the whole population trend by evaluating for each trait the medians and quartiles. As a result, 16 promising isolates were selected and identified by 16S rDNA sequencing (, , , and ). The last step of this research was a preliminary validation in a growth chamber on eight strains. As screening and simple indices, two quantitative measures were chosen. The main result was the selection of at least three isolates (6P, 20P, and 25A) for a future field validation. They increased biomass and height by respectively 50% and 25%.
本文的主要目标是筛选出有潜力作为福贾县硬粒小麦促生菌（PGPB）的微生物。为此，采用了一种基于多变量分析和各性状统计分布评估的新统计框架。从福贾县硬粒小麦根际分离出474株菌株，并根据四个目标指标（铵生产、铁载体生产、磷溶解和硝化作用）进行初步筛选。经过这一步骤，菌株数量减少，剩余的分离株通过定量方法进行测试，以评估吲哚乙酸（IAA）的产生、磷矿化和硝化作用。在第二步中，通过评估每个性状的中位数和四分位数，根据总体趋势确定临界值。结果，通过16S rDNA测序（……）筛选并鉴定出16株有潜力的分离株。本研究的最后一步是在生长室对8株菌株进行初步验证。作为筛选和简单指标，选择了两种定量方法。主要结果是选出至少三株分离株（6P、20P和25A）用于未来的田间验证。它们分别使生物量和株高增加了50%和25%。
