Stratoulias Vassilis, Michon Frederic
Institute of Biotechnology, Helsinki Institute of Life Science, Developmental Biology Program, University of Helsinki, 00790, Helsinki, Finland.
Institute for Neurosciences of Montpellier, INSERM UMR1051, University of Montpellier, 34295, Montpellier, France.
MethodsX. 2019 Oct 17;6:2429-2438. doi: 10.1016/j.mex.2019.10.009. eCollection 2019.
Recent advances in bioengineering and biomaterials, along with knowledge deriving from the fields of developmental biology and stem cell research, have rendered feasible functional replacement of full organs. Here, we describe the methodology for bioengineering a tooth, starting from embryonic epithelial and mesenchymal single cell suspensions. In addition, we describe the subsequent steps of processing this minute structure for use in applications such as histological examination, immunofluorescence and hybridisation. This methodology can be used for any minute structure that needs to be used in paraffin blocks. •Detailed methodology for reproducible and reliable results•Extra step to ensure single cell populations•Subsequent minute structure processing for histological analysis.
生物工程和生物材料领域的最新进展，以及发育生物学和干细胞研究领域的知识，已使全器官的功能性替代成为可能。在此，我们描述了从胚胎上皮和间充质单细胞悬液开始生物工程制造牙齿的方法。此外，我们描述了对这个微小结构进行后续处理以用于组织学检查、免疫荧光和杂交等应用的步骤。这种方法可用于任何需要用于石蜡块的微小结构。•获得可重复和可靠结果的详细方法•确保单细胞群体的额外步骤•用于组织学分析的后续微小结构处理。
