Ingeniería en Innovación Agrícola Sustentable, Instituto Tecnológico Superior de Los Reyes, Los Reyes, Michoacán, México.
Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, México.
Folia Microbiol (Praha). 2020 Jun;65(3):511-521. doi: 10.1007/s12223-019-00755-7. Epub 2019 Nov 13.
Fungi are considered model organisms for the analysis of important phenomena of eukaryotes. For example, some of them have been described as models to understand the phenomenon of multicellularity acquisition by different unicellular organisms phylogenetically distant. Interestingly, in this work, we describe the multicellular development in the model fungus S. reilianum. We observed that Sporisorium reilianum, a Basidiomycota cereal pathogen that at neutral pH grows with a yeast-like morphology during its saprophytic haploid stage, when incubated at acid pH grew in the form of multicellular clusters. The multicellularity observed in S. reilianum was of clonal type, where buds of "stem" cells growing as yeasts remain joined by their cell wall septa, after cytokinesis. The elaboration and analysis of a regulatory network of S. reilianum showed that the putative zinc finger transcription factor CBQ73544.1 regulates a number of genes involved in cell cycle, cellular division, signal transduction pathways, and biogenesis of cell wall. Interestingly, homologous of these genes have been found to be regulated during Saccharomyces cerevisiae multicellular growth. In adddition, some of these genes were found to be negatively regulated during multicellularity of S. reilianum. With these data, we suggest that S. reilianum is an interesting model for the study of multicellular development.
真菌被认为是分析真核生物重要现象的模式生物。例如，其中一些已被描述为模型，用于了解不同亲缘关系的单细胞生物获得多细胞性的现象。有趣的是，在这项工作中，我们描述了模式真菌 S. reilianum 的多细胞发育。我们观察到，Sporisorium reilianum 是一种担子菌门的谷类病原体，在中性 pH 值下，它在其腐生单倍体阶段以酵母样形态生长，而在酸性 pH 值下，它则以多细胞簇的形式生长。在 S. reilianum 中观察到的多细胞性是克隆型的，其中“茎”细胞的芽以酵母形式生长，在细胞分裂后仍通过细胞壁隔膜连接。对 S. reilianum 调控网络的精心分析表明，假定的锌指转录因子 CBQ73544.1 调控了许多参与细胞周期、细胞分裂、信号转导途径和细胞壁生物发生的基因。有趣的是，在酿酒酵母多细胞生长过程中发现了这些基因的同源物。此外，在 S. reilianum 的多细胞性过程中，发现其中一些基因受到负调控。有了这些数据，我们认为 S. reilianum 是研究多细胞发育的一个有趣模型。
