Tóth Katalin, Batek Josef, Stacey Gary
University of Missouri, Division of Plant Sciences, Columbia, Missouri.
University of Missouri, Divisions of Plant Sciences and Biochemistry, National Center for Soybean Biotechnology, Columbia, Missouri.
Curr Protoc Plant Biol. 2016 May;1(1):1-13. doi: 10.1002/cppb.20017.
Legumes-because of their nitrogen-fixing capacity-have both ecological and agronomic importance, and are also the major plant protein source for animal consumption. The model legume species are Lotus japonicus, Medicago truncatula, and soybean (Glycine max). These species have sequenced genomes and are amenable to genetic manipulation, as well as to various functional genomic and cell biology approaches. Plant transformation mediated by Agrobacterium is one of the most powerful methods in plant biotechnology. Using the traditional Agrobacterium tumefaciens method, stable transgenic plants take 6 to 12 months to create, depending on species. Besides being time consuming, this approach is often quite laborious. Hence, there is a need for more rapid methods to create transgenic tissues. In the case of roots, this can be done using hairy root transformation mediated by Agrobacterium rhizogenes. This protocol describes a method to generate transgenic soybean roots in as little as 3 weeks. © 2016 by John Wiley & Sons, Inc.
豆科植物因其固氮能力而具有生态和农艺重要性，也是动物食用的主要植物蛋白来源。豆科模式植物有百脉根、蒺藜苜蓿和大豆（Glycine max）。这些物种的基因组已被测序，适合进行基因操作，以及各种功能基因组学和细胞生物学研究方法。农杆菌介导的植物转化是植物生物技术中最强大的方法之一。使用传统的根癌农杆菌方法，根据物种不同，创建稳定的转基因植物需要6到12个月。这种方法不仅耗时，而且通常相当费力。因此，需要更快速的方法来创建转基因组织。对于根来说，可以使用发根农杆菌介导的毛状根转化来实现。本方案描述了一种在短短3周内生成转基因大豆根的方法。© 2016约翰威立父子公司版权所有。
