Biotechnology Department, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Appl Biochem Biotechnol. 2020 Apr;190(4):1212-1223. doi: 10.1007/s12010-019-03159-y. Epub 2019 Nov 15.
Monogalactosyldiacylglycerols (MGDGs) from microalgae containing a high proportion of polyunsaturated fatty acids (PUFAs) have enormous potential applications in various industries. In this study, the productivity and fatty acid composition of MGDGs from three microalgae in commercialized production, Chlorella sorokiniana GT, Nannochloropsis oceanica IMET1, and Arthrospira platensis, were evaluated under nitrogen-sufficient (+N) and nitrogen-deficient (-N) conditions. Under +N conditions, higher productivities of MGDGs from C. sorokiniana GT1, N. oceanica IMET1, and A. platensis were obtained with 16.3, 4.3, and 1.3 mg/L/day, respectively. In agreement with a high ratio of PUFAs in MGDGs, they accounted for 56%, 66%, and 47% of the total fatty acids in MGDG correspondingly. α-linoleic acid (ALA), eicosapentaenoic acid (EPA), and γ-linoleic acid (GLA) were the specific PUFAs of the three microalgae. The proportions of the specific PUFAs in MGDG accounted for 26%, 44%, and 63% of total specific PUFAs, respectively. Considering the production, cost of microalgae biomass and price of MGDG, the commercialized microalgae C. sorokiniana GT1 and A. platensis showed great potential to produce MGDGs with a high content of PUFAs, in contrast to N. oceanica IMET1, with its high biomass cost and low production. This study provides basic data for the extraction and separation of microalgae lipids from commercialized microalgae, which is helpful to promote the commercialization of microalgae.
单半乳糖二酰基甘油（MGDG）来源于富含多不饱和脂肪酸（PUFA）的微藻，在各个行业具有巨大的潜在应用价值。本研究在氮充足（+N）和氮缺乏（-N）条件下，评估了三种商业化生产的微藻——杜氏盐藻 GT、东海原甲藻 IMET1 和钝顶螺旋藻中 MGDG 的生产力和脂肪酸组成。在+N 条件下，杜氏盐藻 GT1、东海原甲藻 IMET1 和钝顶螺旋藻的 MGDG 生产力分别达到 16.3、4.3 和 1.3mg/L/d。与 MGDG 中高比例的多不饱和脂肪酸相一致，它们分别占 MGDG 总脂肪酸的 56%、66%和 47%。α-亚麻酸（ALA）、二十碳五烯酸（EPA）和γ-亚麻酸（GLA）是这三种微藻的特定多不饱和脂肪酸。三种微藻 MGDG 中特定多不饱和脂肪酸的比例分别占总特定多不饱和脂肪酸的 26%、44%和 63%。考虑到产量、微藻生物量的成本和 MGDG 的价格，与东海原甲藻 IMET1 相比，商业化的杜氏盐藻 GT1 和钝顶螺旋藻具有生产富含多不饱和脂肪酸的 MGDG 的巨大潜力，因为前者的生物量成本高，产量低。本研究为从商业化微藻中提取和分离微藻脂质提供了基础数据，有助于推动微藻的商业化。
