Medical Technology Institute of Xuzhou Medical University, Xuzhou 221004, China; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, No. 99, West Huaihai Road, Xuzhou 221002, China.
Medical Technology Institute of Xuzhou Medical University, Xuzhou 221004, China.
Clin Chim Acta. 2020 Feb;501:252-257. doi: 10.1016/j.cca.2019.10.047. Epub 2019 Nov 12.
Gastric cancer (GC) is one of the most aggressive cancers, with limited early diagnostic measures. Tumor-originated exosomal molecules are regarded as suitable candidates for non-invasive diagnosis. This study aimed to investigate the capacity of exosomal long noncoding RNA lnc-GNAQ-6:1 as a biomarker for early diagnosis of GC.
In this study, we collected sera from 43 patients with gastric cancer and 27 healthy subjects, then exosomes were isolated using commercial kits. Particle size analysis, Western bloting and protein-based exosomes quantification were conducted to identify the isolated exosomes and to evaluate its yield and purity. Expression levels of exosomal lnc-GNAQ-6:1 were detected by quantitative reverse transcription PCR (qRT-PCR). The serum concentrations of traditional biomarker (CA72-4, CEA, and CA19-9) were measured via a chemiluminescent detection system.The receiver operating characteristic curve (ROC) and area under curve (AUC) were used to estimate the diagnostic capacity. Furthermore, we analyzed the potential relationship between serum exosomal lnc-GNAQ-6:1 expression and clinicopathological parameters of gastric cancer.
The exosomes extracted in this study exhibited the typical exosome characteristics and purity. Patients with gastric cancer had the higher exosome yield than healthy volunteer. The results of qRT-PCR showed that compared with the healthy control, the expression of lnc-GNAQ-6:1 was significantly lower in the gastric cancer group. The area under the ROC curve is 0.732, which was higher than the diagnostic accuracy of CEA, CA 19-9 and CA72-4. However, the expression level of lnc-GNAQ-6:1 was not correlated with gender, age, tumor metastasis, serum carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and carbohydrate antigen 72-4(CA72-4).
Our data demonstrated that serum exosomal lnc-GNAQ-6:1 is lowly expressed in patient with gastric cancer and might be evaluated in larger studies as a new diagnostic marker for gastric cancer.
胃癌（GC）是最具侵袭性的癌症之一，早期诊断措施有限。肿瘤来源的外泌体分子被认为是一种适合非侵入性诊断的候选物。本研究旨在探讨外泌体长非编码 RNA lnc-GNAQ-6:1 作为 GC 早期诊断生物标志物的能力。
本研究收集了 43 例胃癌患者和 27 例健康受试者的血清，然后使用商业试剂盒分离外泌体。通过颗粒大小分析、Western blot 和基于蛋白质的外泌体定量来鉴定分离的外泌体，并评估其产量和纯度。通过定量逆转录 PCR（qRT-PCR）检测外泌体 lnc-GNAQ-6:1 的表达水平。通过化学发光检测系统测量传统生物标志物（CA72-4、CEA 和 CA19-9）的血清浓度。使用受试者工作特征曲线（ROC）和曲线下面积（AUC）来评估诊断能力。此外，我们分析了血清外泌体 lnc-GNAQ-6:1 表达与胃癌临床病理参数之间的潜在关系。
本研究提取的外泌体表现出典型的外泌体特征和纯度。胃癌患者的外泌体产量高于健康志愿者。qRT-PCR 结果显示，与健康对照组相比，胃癌组 lnc-GNAQ-6:1 的表达明显降低。ROC 曲线下面积为 0.732，高于 CEA、CA19-9 和 CA72-4 的诊断准确性。然而，lnc-GNAQ-6:1 的表达水平与性别、年龄、肿瘤转移、血清癌胚抗原（CEA）、糖链抗原 19-9（CA19-9）和糖链抗原 72-4（CA72-4）无关。
我们的数据表明，胃癌患者血清外泌体 lnc-GNAQ-6:1 表达水平较低，可能在更大的研究中作为胃癌的新诊断标志物进行评估。
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