Superintendent Office, Mackay Memorial Hospital, Taipei, Taiwan.
Department of Medical Laboratory Science and Biotechnology, Yuanpei University of Medical Technology, Hsinchu, Taiwan.
J Clin Lab Anal. 2020 Apr;34(4):e23110. doi: 10.1002/jcla.23110. Epub 2019 Nov 15.
Despite having chronic gastritis, most people infected by Helicobacter pylori (H. pylori) are asymptomatic and have no specific clinical signs and symptoms. H. pylori infection can be diagnosed by several detection methods. Giemsa stain and rapid urease test (CLO test) are the most performed tests of H. pylori infection at first-line clinical examination because of their simplicity and reliability. However, the sensitivity of CLO test is significantly reduced in patients with atrophic gastritis and intestinal metaplasia, and the weaknesses of Giemsa stain are higher cost and time-consuming.
The Giemsa stain was modified in several staining solutions and procedures based on the simplified Giemsa technique described by Gray, Wyatt, & Rathbone (1986). The modified Giemsa stain is examined its efficacy and compared with the CLO test using 233 H. pylori-infected patients with gastric disease.
The modified Giemsa stain is comparable to the traditional one. Statistical analysis indicated that the modified Giemsa stain obtains greater accuracy in H. pylori-infected patients with gastritis and ulcer than the CLO test (48.1% vs. 43.7%). Moreover, considering the prognosis of different symptoms of gastric diseases, the modified Giemsa stain has a more accurate prognosis than combination symptoms (P = 1.8E-05 vs. P = 5.49E-05). The modified Giemsa stain is confirmed to be better than CLO test using 233 H. pylori-infected patients with gastric disease.
The modified Giemsa stain is more simplified and time-saving than traditional Giemsa stain, which is comparable to the traditional one and is confirmed to be better than CLO test using 233 H. pylori-infected patients with gastric disease. In clinical examination, this modified Giemsa stain can be applied to routine examination and provides quick and accurate diagnosis and prognosis to H. pylori-infected patients with gastric diseases.
尽管患有慢性胃炎，大多数感染幽门螺杆菌 (H. pylori) 的人无症状且无特定临床体征和症状。H. pylori 感染可通过几种检测方法诊断。由于其简单性和可靠性，革兰氏染色和快速尿素酶试验（CLO 试验）是一线临床检查中最常进行的 H. pylori 感染检测方法。然而，在萎缩性胃炎和肠上皮化生患者中，CLO 试验的敏感性显著降低，革兰氏染色的弱点是成本较高且耗时较长。
根据 Gray、Wyatt 和 Rathbone（1986 年）描述的简化革兰氏染色技术，对几种染色溶液和程序进行了革兰氏染色的改良。改良的革兰氏染色与 CLO 试验一起检查其疗效，并使用 233 例患有胃病的 H. pylori 感染患者进行比较。
改良的革兰氏染色与传统的革兰氏染色相当。统计分析表明，改良的革兰氏染色在胃炎和溃疡的 H. pylori 感染患者中比 CLO 试验获得更高的准确性（48.1%比 43.7%）。此外，考虑到不同胃病症状的预后，改良的革兰氏染色比联合症状具有更准确的预后（P=1.8E-05 比 P=5.49E-05）。使用 233 例患有胃病的 H. pylori 感染患者证实，改良的革兰氏染色优于 CLO 试验。
改良的革兰氏染色比传统的革兰氏染色更简化和省时，与传统的革兰氏染色相当，并使用 233 例患有胃病的 H. pylori 感染患者证实优于 CLO 试验。在临床检查中，这种改良的革兰氏染色可用于常规检查，为患有胃病的 H. pylori 感染患者提供快速准确的诊断和预后。
