Arias E
Dipartimento di Biologia, Università di Milano, Italy.
Mutat Res. 1988 Oct;206(2):271-3. doi: 10.1016/0165-1218(88)90170-x.
The genotoxic potential of a commercial formulation of the fungicide maneb (Maneb 80, containing 80% manganese ethylenebisdithiocarbamate as active ingredient) in chick embryos was evaluated, using as genetic end-points the frequency of sister-chromatid exchanges (SCE) and of chromosomal aberrations. Unincubated eggs were dipped in 0, 0.5, 1.5, 4.5, 13.5 or 27 g/l maneb aqueous solutions for 30 sec. Eggs were then incubated for 4 days. Maneb significantly increased SCE values at 13.5 g/l and at 27 g/l (means 1.33 and 1.74) over the control value (mean 0.87). The concentration of 27 g/l, which corresponds to 10.8 times the recommended maximum application level for use in the field, also resulted in a high mortality rate. No clastogenic effects following exposure to maneb were observed.
使用姐妹染色单体交换（SCE）频率和染色体畸变作为遗传终点，评估了杀菌剂代森锰锌（代森锰锌80，含有80%的乙撑双二硫代氨基甲酸锰作为活性成分）商业制剂对鸡胚的遗传毒性潜力。将未孵化的鸡蛋浸入0、0.5、1.5、4.5、13.5或27 g/l的代森锰锌水溶液中30秒。然后将鸡蛋孵化4天。代森锰锌在13.5 g/l和27 g/l时显著增加了SCE值（平均值分别为1.33和1.74），高于对照值（平均值0.87）。27 g/l的浓度相当于田间推荐最大施用水平的10.8倍，也导致了高死亡率。未观察到接触代森锰锌后的致断裂效应。
