帕金森病患者的铜、铁、锌和锰的状况和形态评估：一项初步研究。

Assessment of copper, iron, zinc and manganese status and speciation in patients with Parkinson's disease: A pilot study.

机构信息

I.M. Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya St., 8-2, 119991 Moscow, Russia; Peoples' Friendship University of Russia (RUDN University), Miklukho-Maklay St., 10/2, Moscow 117198, Russia; Federal Scientific Center of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 9 Yanvarya St., 29, 460000 Orenburg, Russia.

I.M. Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya St., 8-2, 119991 Moscow, Russia; P.G. Demidov Yaroslavl State University, Sovetskaya st., 14, 150003 Yaroslavl, Russia; Federal Scientific Center of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 9 Yanvarya St., 29, 460000 Orenburg, Russia.

出版信息

J Trace Elem Med Biol. 2020 May;59:126423. doi: 10.1016/j.jtemb.2019.126423. Epub 2019 Oct 28.

DOI:10.1016/j.jtemb.2019.126423

PMID:31733982

Abstract

BACKGROUND

The objective of this pilot study was to assess iron (Fe), copper (Cu), zinc (Zn), and manganese (Mn) status (hair, serum, and urine) and speciation (serum) in Parkinson's disease (PD) patients.

METHODS

A pilot study involving a total of 27 subjects (13 PD patients, 14 controls) was performed. Serum, urine, and hair metal content was assessed using ICP-MS. Speciation analysis of Cu, Zn, Fe, and Mn was performed using a hybrid HPLC-ICP-MS system.

RESULTS

Group comparisons did not reveal any significant group difference in serum Cu, Zn, Fe, and Mn total metal level between PD patients and controls. Speciation analysis revealed a significant decrease in Cu/ceruloplasmin copper in association with elevation of low-molecular weight species (amino acids)-bound copper. It is proposed that in PD, binding of Cu(II) ions to ceruloplasmin is reduced and free copper ions coordinate with low molecular weight ligands. The level of Mn-albumin complexes in PD patients was more than 4-fold higher as compared to the respective value in the control group. The observed difference may be considered as a marker of redistribution between high and low molecular weight ligands.

CONCLUSIONS

Metal speciation is significantly affected in serum of PD-patients. These findings are indicative of the potential role of metal metabolism and PD pathogenesis, although the exact mechanisms of such associations require further detailed studies.

摘要

背景

本初步研究旨在评估帕金森病（PD）患者的铁（Fe）、铜（Cu）、锌（Zn）和锰（Mn）状态（头发、血清和尿液）和形态（血清）。

方法

共进行了一项涉及 27 名受试者（13 名 PD 患者，14 名对照）的初步研究。使用 ICP-MS 评估血清、尿液和头发中的金属含量。使用 HPLC-ICP-MS 系统对 Cu、Zn、Fe 和 Mn 的形态进行分析。

结果

组间比较未发现 PD 患者与对照组之间血清 Cu、Zn、Fe 和 Mn 总金属水平存在任何显著差异。形态分析显示 Cu/铜蓝蛋白铜的显著减少与低分子量物质（氨基酸）结合的铜升高有关。据推测，在 PD 中，Cu(II)离子与铜蓝蛋白的结合减少，游离的铜离子与低分子量配体配位。与对照组相比，PD 患者的 Mn-白蛋白复合物水平高出 4 倍以上。观察到的差异可被认为是高和低分子量配体之间重新分布的标志物。