Department of Microbiology, Immunology and Biopharmaceuticals, National Chiayi University, Chiayi City, Taiwan.
Institute of Preventive Medicine, National Defense Medical Center, New Taipei City, Taiwan.
Int J Biol Macromol. 2020 Jan 15;143:724-731. doi: 10.1016/j.ijbiomac.2019.10.002. Epub 2019 Nov 15.
Herein, positively surface-charged silver nanoparticles (AgNPs) capped with trimethylchitosan nitrate (TMCN) were synthesized using an environmentally friendly method. Nano-sized TMCN-AgNPs (~80 nm) with high zeta potential (>30 mV) provide sufficient static repulsion to stabilize colloid AgNPs in aqueous solutions without aggregation for >3 months. In in vitro cell cycle assays, TMCN-AgNPs showed low cytotoxicity towards L929 cells. A microdilution inhibition assay demonstrated the antifungal potential of TMCN-AgNPs, with a minimum inhibitory concentration of 0.06 mM against Candida tropicalis ATCC 750, and 0.46 mM against both Candida albicans ATCC 76615 and Candida glabrata ATCC 15545. Moreover, the addition of TMCN-AgNPs at 0.23 mM significantly reduced biofilm formation in 96-well plates with C. albicans and C. tropicalis. Importantly, when zebrafish eggs were infected with Candida cells, 0.23 mM TMCN-AgNPs greatly diminished the amount of biofilm on eggs and rescued the survival of embryos by up to 70%.
本文采用环保方法合成了带正电荷的硝酸三甲基壳聚糖（TMCN）包覆的银纳米颗粒（AgNPs）。具有高 zeta 电位（>30 mV）的纳米级 TMCN-AgNPs（~80 nm）提供了足够的静电斥力，可使胶体 AgNPs 在水溶液中稳定 3 个月以上而不聚集。在体外细胞周期试验中，TMCN-AgNPs 对 L929 细胞的细胞毒性较低。微量稀释抑制试验表明 TMCN-AgNPs 具有抗真菌潜力，对热带假丝酵母 ATCC 750 的最小抑菌浓度为 0.06 mM，对白色假丝酵母 ATCC 76615 和光滑假丝酵母 ATCC 15545 的最小抑菌浓度分别为 0.46 mM。此外，在 96 孔板中添加 0.23 mM 的 TMCN-AgNPs 可显著减少白色假丝酵母和热带假丝酵母的生物膜形成。重要的是，当斑马鱼卵被念珠菌细胞感染时，0.23 mM 的 TMCN-AgNPs 可大大减少卵上生物膜的数量，并使胚胎的存活率提高 70%。
