Artunduaga Bonilla Jhon J, Paredes Guerrero Daissy J, Sánchez Suárez Clara I, Ortiz López Claudia C, Torres Sáez Rodrigo G
Facultad de Salud, Escuela de Microbiología, Universidad Industrial de Santander, Bucaramanga, 680002, Colombia.
Facultad de Ciencias, Escuela de Química, Universidad Industrial de Santander, Bucaramanga, 680002, Colombia.
World J Microbiol Biotechnol. 2015 Nov;31(11):1801-9. doi: 10.1007/s11274-015-1933-z. Epub 2015 Sep 3.
Nowadays, current advances in nanotechnology constitute a promising alternative in the development of new antimicrobial agents. Silver nanoparticles (AgNPs) are some very interesting products currently provided by available nanotechnology for control of microbial infection. In the present study, AgNPs were synthesized by eco-friendly method, using cysteine as a reducing agent. Also, antifungal activity against Candida species with resistance to fluconazole was evaluated through determination of Minimum Inhibitory Concentration (MIC50) according to protocol M27-A3 of Clinical and Laboratory Standards Institute (CLSI) and Minimum Fungicide Concentration (MFC). This study was carried out with strains Candida krusei and Candida glabrata. As a result, the formation of spherical nanoparticles was obtained with mean sizes of 19 nm and positive surface charge. Values of MIC50 were 0.1 µg ml(-1) AgNPs for the studied species, and MFC were 0.25 and 0.5 µg ml(-1) for C. glabrata and C. krusei, respectively. The AgNPs synthesized showed cytotoxic effect in 50% of Murine Fibroblast Cells (CC50) at a mean concentrations of 10 µg ml(-1) (100 times higher than MIC50). Consequently, AgNPs could be considered as an alternative potential in the development of new antifungal agents with minimum cytotoxicity in fibroblasts and lethal action on Candida species with resistance to conventional antifungal compounds.
如今，纳米技术的当前进展为新型抗菌剂的开发提供了一个有前景的选择。银纳米颗粒（AgNPs）是现有纳米技术目前提供的一些非常有趣的用于控制微生物感染的产品。在本研究中，采用环保方法，以半胱氨酸作为还原剂合成了AgNPs。此外，根据临床和实验室标准协会（CLSI）的M27 - A3方案，通过测定最低抑菌浓度（MIC50）和最低杀菌浓度（MFC），评估了对氟康唑耐药的念珠菌属的抗真菌活性。本研究使用了克鲁斯念珠菌和光滑念珠菌菌株。结果，获得了平均尺寸为19 nm且表面带正电荷的球形纳米颗粒。所研究物种的MIC50值为0.1 µg ml(-1) AgNPs，光滑念珠菌和克鲁斯念珠菌的MFC分别为0.25和0.5 µg ml(-1)。合成的AgNPs在平均浓度为10 µg ml(-1)（比MIC50高100倍）时，对50%的小鼠成纤维细胞（CC50）显示出细胞毒性作用。因此，AgNPs可被视为开发新型抗真菌剂的一种潜在选择，其对成纤维细胞的细胞毒性最小，且对耐传统抗真菌化合物的念珠菌具有致死作用。
