Medical Mycology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, India; Enzyme Technology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
Medical Mycology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
J Mycol Med. 2019 Sep;29(3):201-209. doi: 10.1016/j.mycmed.2019.07.005. Epub 2019 Jul 26.
Candida albicans is a polymorphic opportunistic commensal that causes both superficial and systemic fungal infections especially in immunocompromised patients. Biologically synthesized silver nanoparticles (AgNPs) have emerged as potential antifungal agents. The present work evaluates the antifungal activity of Artemisia annua synthesized AgNPs against three Candida species (C. albicans ATCC 90028, C. tropicalis ATCC 750 and C. glabrata ATCC 90030). The in vitro effect of AgNPs was investigated for fungal growth, sterol content, secretion of hydrolytic enzymes and yeast-to-hyphal transition. The green synthesized AgNPs were effective against all the three species with minimum inhibitory concentration (MIC) in the range 80-120μgml. Candida glabrata showed greater sensitivity for AgNPs followed by Candida tropicalis and C. albicans. AgNPs at 4MIC were as effective as fluconazole (FLC) and caused only 5% haemolysis while FLC caused 50% haemolysis at the same concentration. The secretion of hydrolytic enzymes was the lowest in case of AgNP exposed C. glabrata. Yeast-to-hyphal transition was significantly reduced in treated C. albicans cells and showed disfigured morphology in SEM images. The decrease in ergosterol content was slightly higher (94%) in both C. glabrata and C. tropicalis in comparison to C. albicans (69%). Green synthesized AgNPs thus have immense potential as an antifungal and can play a crucial role in the management of Candida infections especially those caused by C. glabrata.
白色念珠菌是一种多态性机会性共生菌，可引起浅表和系统性真菌感染，尤其在免疫功能低下的患者中。生物合成的银纳米粒子（AgNPs）已成为有前途的抗真菌药物。本研究评估了青蒿素合成的 AgNPs 对三种念珠菌（白色念珠菌 ATCC 90028、热带念珠菌 ATCC 750 和光滑念珠菌 ATCC 90030）的抗真菌活性。研究了 AgNPs 对真菌生长、甾醇含量、水解酶分泌和酵母-菌丝过渡的体外作用。绿色合成的 AgNPs 对所有三种真菌均有效，最低抑菌浓度（MIC）范围为 80-120μgml。光滑念珠菌对 AgNPs 的敏感性最高，其次是热带念珠菌和白色念珠菌。AgNPs 在 4MIC 时与氟康唑（FLC）一样有效，仅引起 5%的溶血，而相同浓度的 FLC 引起 50%的溶血。AgNP 暴露的 C. glabrata 中水解酶的分泌最低。处理后的 C. albicans 细胞中的酵母-菌丝过渡明显减少，并且在 SEM 图像中显示出畸形形态。与白色念珠菌（69%）相比，光滑念珠菌和热带念珠菌中麦角固醇含量的下降略高（94%）。因此，绿色合成的 AgNPs 具有巨大的抗真菌潜力，可以在念珠菌感染的治疗中发挥关键作用，尤其是由 C. glabrata 引起的感染。
