Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Food Chem. 2020 May 1;311:125874. doi: 10.1016/j.foodchem.2019.125874. Epub 2019 Nov 9.
The Lycium ruthenicum (Lr) fruit is a widely used nutritional food that contains various bioactive components such as anthocyanin and spermidine derivatives. In the present study, ultra-high-performance liquid chromatography with tandem mass spectrometry was utilized to profile the metabolic dynamics of four developmental stages of Lr fruit. A total of 49 compounds, including anthocyanin, alkaloids, hydroxycinnamic acid derivatives, flavonoids, and amino acids, were tentatively identified. Principal component analysis distinguished the fruit at four developmental stages using 15 (9 were tentatively identified) potential marker compounds. Pearson correlation analysis suggested that anthocyanin and spermidine derivative hexoses had a strong positive correlation coefficient. A glucosyltransferase (HG27071) was confirmed to glucosylate both anthocyanidin and spermidine derivative in vitro. Our results provide insight into the metabolic linkages among bioactive components in Lr fruits. The glucosyltransferase identified in this study will promote its potential use in functional foods and natural pigment resources.
黑果枸杞是一种广泛应用的营养食品，其果实含有多种生物活性成分，如花色苷和亚精胺衍生物。本研究采用超高效液相色谱-串联质谱法对黑果枸杞果实四个发育阶段的代谢动态进行了分析。共鉴定出 49 种化合物，包括花色苷、生物碱、羟基肉桂酸衍生物、类黄酮和氨基酸。主成分分析用 15 种（其中 9 种为暂定）潜在标志物化合物区分了四个发育阶段的果实。Pearson 相关分析表明花色苷和亚精胺衍生物己糖具有很强的正相关系数。体外实验证实，葡萄糖基转移酶（HG27071）能够对花色苷和亚精胺衍生物进行葡萄糖基化。本研究结果为黑果枸杞果实中生物活性成分之间的代谢关系提供了深入的见解。本研究中鉴定的葡萄糖基转移酶将促进其在功能性食品和天然色素资源中的潜在应用。
