Childress William M, Bosworth Brian, Chesney Edward, Walter Ronald B, Tiersch Terrence R
Aquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, 2288 Gourrier Avenue, Baton Rouge, LA 70820, USA.
Warmwater Aquaculture Research Unit, USDA-ARS, P.O. Box 38, Stoneville, MS 38776, USA.
N Am J Aquac. 2019 Oct;81(4):349-363. doi: 10.1002/naaq.10099. Epub 2019 May 20.
Cryopreservation of genetic material can become an important tool for user groups in imperiled fishes, wild fisheries, aquaculture, and biomedical research. Persistent challenges within aquatic species cryopreservation are standardization and reliable collection of diverse, high quality samples. The overall goal of this study was to work with different user groups and cryopreserve sperm on-site at their facilities to evaluate the uses and challenges of a mobile laboratory with high-throughput and quality control capabilities comparable to those of a specialized centralized facility. The objectives were to demonstrate collection and cryopreservation of sperm of: 1) large-bodied freshwater Blue Catfish () for aquaculture; 2) small-bodied freshwater for biomedical and imperiled repository development, and 3) saltwater Red Snapper () for wild fisheries research. Over the course of this project, the mobile laboratory traveled more than 4,000 km collecting germplasm from more than 650 male fishes. A total of 136 Blue Catfish were processed in 2015 and 2016 resulting in a total of 6,146 0.5-mL French straws. A total of 521 males from 11 different species in the genus were processed over 4 d in 2015 resulting in a total of 488 0.25-mL French straws. And, a total of 17 Red Snapper males were processed during 2015 resulting in a total of 316 0.5-mL French straws. This is the first development of a mobile laboratory with high-throughput capability for aquatic species. User groups would no longer be limited to germplasm resources that can only be shipped as samples or transported as live animals to a central cryopreservation facility. Mobile laboratories create opportunities to collect higher quality germplasm, provide access to new species, and enable direct cooperation, including training, with a wide variety of user groups and applications.
遗传物质的冷冻保存对于濒危鱼类、野生渔业、水产养殖和生物医学研究等用户群体而言，可能会成为一项重要工具。水生物种冷冻保存中一直存在的挑战是标准化以及可靠地采集多样的高质量样本。本研究的总体目标是与不同用户群体合作，在其设施现场对精子进行冷冻保存，以评估一个具有与专业集中设施相当的高通量和质量控制能力的移动实验室的用途和挑战。目标是展示对以下物种精子的采集和冷冻保存：1）用于水产养殖的大型淡水蓝鲶（ ）；2）用于生物医学和濒危物种库开发的小型淡水 ；3）用于野生渔业研究的海水红鲷（ ）。在本项目过程中，移动实验室行程超过4000公里，从650多条雄鱼采集种质。2015年和2016年共处理了136条蓝鲶，共得到6146支0.5毫升法式细管。2015年在4天内对 属11个不同物种的521条雄鱼进行了处理，共得到488支0.25毫升法式细管。并且，2015年共处理了17条红鲷雄鱼，共得到316支0.5毫升法式细管。这是首个针对水生物种开发的具有高通量能力的移动实验室。用户群体将不再局限于只能作为样本运输或作为活体动物运输到中央冷冻保存设施的种质资源。移动实验室创造了采集更高质量种质的机会，提供了接触新物种的途径，并能够与广泛的用户群体和应用领域进行包括培训在内的直接合作。
