Amladi A, Devanga Ragupathi N K, Vasudevan K, Venkatesan M, Anandan S, Veeraraghavan B
Department of Clinical Microbiology, Christian Medical College, Vellore, 632 004, Tamil Nadu, India.
New Microbes New Infect. 2019 Oct 15;32:100613. doi: 10.1016/j.nmni.2019.100613. eCollection 2019 Nov.
Melioidosis caused by has become an important clinical threat, especially in Northern Australia and Southeast Asia. However, the genome information on this pathogen is limited. isolates identified from bloodstream infections from inpatients were subjected to whole-genome sequencing by IonTorrent PGM and MinION Oxford Nanopore sequencing technologies. Highly accurate complete genomes of two strains, VB3253 and VB2514, were obtained by a hybrid genome assembly method using both short and long DNA reads. Both isolates carried PenI and carbapenemase-encoding OXA-57 genes, although the isolates were susceptible to imipenem by E-test method with MIC 1 μg/mL. Multiple IS family transposases specific for all non-fermenting Gram-negative bacteria (NFGNBs)-especially IS3 and IS5, which facilitate mobilization of extended-spectrum β-lactamase (ESBL) and carbapenemase genes-were carried in these genomes. This further adds to the complexity of gene transmission. These IS families were identified only upon hybrid genome assembly and would otherwise be missed.
由[病原体名称未给出]引起的类鼻疽已成为一种重要的临床威胁，尤其是在澳大利亚北部和东南亚地区。然而，关于这种病原体的基因组信息有限。对从住院患者血液感染中分离出的[病原体名称未给出]菌株，采用IonTorrent PGM和MinION Oxford Nanopore测序技术进行全基因组测序。通过使用短DNA读段和长DNA读段的混合基因组组装方法，获得了两株菌株VB3253和VB2514的高精度完整基因组。尽管通过E-test法检测，这两株菌株对亚胺培南敏感，MIC为1μg/mL，但它们都携带PenI和编码碳青霉烯酶的OXA-57基因。这些基因组中携带了多种对所有非发酵革兰氏阴性菌（NFGNBs）特异的IS家族转座酶，尤其是IS3和IS5，它们有助于超广谱β-内酰胺酶（ESBL）和碳青霉烯酶基因的移动。这进一步增加了基因传播的复杂性。这些IS家族仅在混合基因组组装时才被鉴定出来，否则将会遗漏。
