Panya Marutpong, Thirat Suthinee, Wanram Surasak, Panomket Pawana, Nilsakul Jiraporn
J Med Assoc Thai. 2016 Jan;99 Suppl 1:S12-6.
Burkholderia pseudomallei is a causative agent of melioidosis. Ceftazidime is the preferred drug of choice for treatment. However, the motility rate is high in endemic areas.
This study aimed to determine the susceptibility tofour different antimicrobial agents and to detect the β-lactamase genes in B. pseudomallei isolates from patients admitted to Sappasitthiprasong Hospital.
85 B. pseudomallei isolates from patients admitted to Sappasitthiprasong Hospital between November 2010 and May 2011 were determined for antimicrobial susceptibility by standard disk diffusion and minimum inhibitory concentration (MIC). Real-time polymerase chain reaction (PCR) was used for the detection of bla(penA) and bla(OXA) in β-lactamase genes.
Almost all of the clinical isolates ofB. pseudomallei were susceptible to ceftazidime and imipenem. Cefatzidime MIC was ≤ 1-16 μg/ml and imipenem MIC was ≤ 1-4 μg/ml. The real-time PCR revealed that more than 90% of B. pseudomallei isolates carried bla(PenA) and bla(OXA).
Although the clinical isolates of B. pseudomallei were susceptible to ceftazidime and imipenem, this study showed B. pseudomallei had a gene that produced beta-lactamase enzyme and may be poorly effective in the use of beta-lactam drugs.
类鼻疽伯克霍尔德菌是类鼻疽的病原体。头孢他啶是治疗的首选药物。然而，在流行地区的菌株耐药率较高。
本研究旨在确定来自Sappasitthiprasong医院患者的类鼻疽伯克霍尔德菌分离株对四种不同抗菌药物的敏感性，并检测β-内酰胺酶基因。
对2010年11月至2011年5月期间入住Sappasitthiprasong医院的患者的85株类鼻疽伯克霍尔德菌分离株，采用标准纸片扩散法和最低抑菌浓度（MIC）测定抗菌药物敏感性。采用实时聚合酶链反应（PCR）检测β-内酰胺酶基因中的bla(penA)和bla(OXA)。
几乎所有类鼻疽伯克霍尔德菌临床分离株对头孢他啶和亚胺培南敏感。头孢他啶MIC≤1-16μg/ml，亚胺培南MIC≤1-4μg/ml。实时PCR显示，超过90%的类鼻疽伯克霍尔德菌分离株携带bla(PenA)和bla(OXA)。
尽管类鼻疽伯克霍尔德菌临床分离株对头孢他啶和亚胺培南敏感，但本研究表明类鼻疽伯克霍尔德菌具有产生β-内酰胺酶的基因，可能对β-内酰胺类药物疗效不佳。
