离子对大鼠肝脏β-半乳糖苷酶活性的刺激作用。
Stimulation of rat liver beta-galactosidase activity by ions.
作者信息
Baccino F M, Zuretti M F, Pernigotti L
出版信息
Biochem J. 1975 Dec;151(3):567-73. doi: 10.1042/bj1510567.
Abstract
- The p-nitrophenyl beta-D-galactosidase asctivity in rat liver homogenates of lysosome-rich fractions was shown to be markedly affected by the ionic composition of the medium. A stimulation of the reaction rate at pH 5 was produced by most of the salts tested, which contained anions such as acetate, SO4(2-) and Cl-, and cations such as Na+, K= and Mg2+. The most pronounced effect was observed with MgCl2. Only potassium glutamate was inhibitory. 2. Five peaks of beta-galactosidase activity obtained by DEAE-cellulose chromatography were equally sensitive to changes in the ionic composition of the medium. In the presence of added NaC1, the whole rate-pH curve was displaced towards higher pH values, the optimum being shifted from 2.0-2.5 to 3.5. The stimulation at pH 5.0 appeared to be mainly due to changes in Vmax., whereas the apparent Km was slightly modified. 3. Unlike the total, the free beta-galactosidase activity remained unchanged or even declined when KC1 was added to the reaction medium.
摘要
- 结果显示，富含溶酶体组分的大鼠肝脏匀浆中的对硝基苯基β-D-半乳糖苷酶活性受到培养基离子组成的显著影响。在pH 5时，大多数测试盐类可提高反应速率，这些盐类含有醋酸根、硫酸根（2-）和氯离子等阴离子，以及钠离子、钾离子和镁离子等阳离子。其中，氯化镁的效果最为显著。只有谷氨酸钾具有抑制作用。2. 通过DEAE-纤维素色谱法获得的五个β-半乳糖苷酶活性峰对培养基离子组成的变化同样敏感。在添加氯化钠的情况下，整个速率-pH曲线向更高pH值方向移动，最适pH值从2.0 - 2.5移至3.5。在pH 5.0时的刺激作用似乎主要是由于Vmax的变化，而表观Km略有改变。3. 与总β-半乳糖苷酶不同，当向反应介质中添加氯化钾时，游离β-半乳糖苷酶活性保持不变甚至下降。
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