吡啶吲哚洛尔对β-半乳糖苷酶的抑制活性。
Inhibitory activity of pyridindolol on beta-galactosidase.
作者信息
Kumagai M, Aoyagi T, Umezawa H
出版信息
J Antibiot (Tokyo). 1976 Jul;29(7):696-703. doi: 10.7164/antibiotics.29.696.
The activity of pyridindolol in inhibiting beta-galactosidases obtained from various sources has been studied. Whereas acid bovine liver beta-galactosidase (optimal pH 4.0) was not affected by this compound, neutral bovine liver beta-galactosidase (pH-optimum = 7.0) was inhibited by pyridindolol in reaction mixtures of pH 4.0 approximately 5.0. There was no inhibition at pH 7.0. The type of inhibition is non-competitive by formation of a pyridindolol-enzyme complex. Since beta-galactosidases from other sources are not affected by pyridindolol, the inhibitory action of this compound seems to be rather specific for neutral bovine liver beta-galactosidase.
已对吡啶吲哚洛尔抑制从各种来源获得的β-半乳糖苷酶的活性进行了研究。酸性牛肝β-半乳糖苷酶（最适pH 4.0）不受该化合物影响，而中性牛肝β-半乳糖苷酶（最适pH = 7.0）在pH 4.0至约5.0的反应混合物中被吡啶吲哚洛尔抑制。在pH 7.0时无抑制作用。抑制类型为通过形成吡啶吲哚洛尔 - 酶复合物的非竞争性抑制。由于来自其他来源的β-半乳糖苷酶不受吡啶吲哚洛尔影响，该化合物的抑制作用似乎对中性牛肝β-半乳糖苷酶具有相当的特异性。
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