Jia Yuan-Ling, Liu Xiao-Jing, Wen Hang, Zhan Yue-Ping, Xiang Min-Hong
Department of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China.
Int J Ophthalmol. 2019 Nov 18;12(11):1801-1806. doi: 10.18240/ijo.2019.11.21. eCollection 2019.
This study investigated the potential role of MAPK signaling pathways in conjunctivochalasis (CCH). Twenty loose conjunctival biopsy samples from 20 CCH and 15 conjunctival biopsy samples from 15 normal controls (CON) were collected. The conjunctival fibroblasts were cultured . Immunofluorescence, ELISA, Western blot and reverse transcription-polymerase chain reaction (RT-PCR) were used. Our results showed that the expression of p-ERK, p-JNK, and p-p38 in CCH conjunctiva was significantly higher than that in CON group. The expression of p38 MAPK, JNK, and ERK proteins in CCH fibroblasts was significantly higher than that in CON group. The total expression of MAPK mRNA in CCH fibroblasts was significantly higher than that in CON group. The activated forms of p38 MAPK, JNK, and ERK proteins and mRNAs might up-regulate the expression of MMPs in CCH loose conjunctival tissue and fibroblasts, causing the degradation of collagen fibers and elastic fibers and promoting the occurrence of CCH. Our results deepen the understanding of CCH pathological mechanism.
本研究调查了丝裂原活化蛋白激酶（MAPK）信号通路在结膜松弛症（CCH）中的潜在作用。收集了来自20例CCH患者的20份结膜活检组织样本以及来自15例正常对照（CON）的15份结膜活检组织样本。对结膜成纤维细胞进行培养，并采用免疫荧光、酶联免疫吸附测定（ELISA）、蛋白质印迹法和逆转录-聚合酶链反应（RT-PCR）。我们的结果显示，CCH结膜中p-ERK、p-JNK和p-p38的表达显著高于CON组。CCH成纤维细胞中p38 MAPK、JNK和ERK蛋白的表达显著高于CON组。CCH成纤维细胞中MAPK mRNA的总表达显著高于CON组。p38 MAPK、JNK和ERK蛋白及mRNA的活化形式可能上调CCH疏松结膜组织和成纤维细胞中基质金属蛋白酶（MMPs）的表达，导致胶原纤维和弹性纤维降解，促进CCH的发生。我们的结果加深了对CCH病理机制的理解。
