Hönig Vaclav, Palus Martin, Kaspar Tomas, Zemanova Marta, Majerova Karolina, Hofmannova Lada, Papezik Petr, Sikutova Silvie, Rettich Frantisek, Hubalek Zdenek, Rudolf Ivo, Votypka Jan, Modry David, Ruzek Daniel
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic.
Department of Virology, Veterinary Research Institute, 62100 Brno, Czech Republic.
Microorganisms. 2019 Nov 16;7(11):568. doi: 10.3390/microorganisms7110568.
Usutu virus (USUV) is a flavivirus (: ) of an African origin transmitted among its natural hosts (diverse species of birds) by mosquitoes. The virus was introduced multiple times to Europe where it caused mortality of blackbirds () and certain other susceptible species of birds. In this study, we report detection of USUV RNA in blackbirds, and mosquitoes in the Czech Republic, and isolation of 10 new Czech USUV strains from carcasses of blackbirds in cell culture. Multiple lineages (Europe 1, 2 and Africa 3) of USUV were found in blackbirds and mosquitoes in the southeastern part of the country. A single USUV lineage (Europe 3) was found in Prague and was likely associated with increased mortalities in the local blackbird population seen in this area in 2018. USUV genomic RNA (lineage Europe 2) was detected in a pool of mosquitoes from South Bohemia (southern part of the country), where no major mortality of birds has been reported so far, and no flavivirus RNA has been found in randomly sampled cadavers of blackbirds. The obtained data contributes to our knowledge about USUV genetic variability, distribution and spread in Central Europe.
乌苏图病毒（USUV）是一种起源于非洲的黄病毒（: ），通过蚊子在其自然宿主（多种鸟类）之间传播。该病毒多次传入欧洲，在欧洲导致了黑鹂（）和其他一些易感鸟类的死亡。在本研究中，我们报告了在捷克共和国的黑鹂、和蚊子中检测到USUV RNA，并从黑鹂尸体中在细胞培养物中分离出10株新的捷克USUV毒株。在该国东南部的黑鹂和蚊子中发现了多个USUV谱系（欧洲1、2和非洲3）。在布拉格发现了一个单一的USUV谱系（欧洲3），这可能与2018年该地区当地黑鹂种群死亡率增加有关。在该国南部的南波希米亚地区的一组蚊子中检测到了USUV基因组RNA（欧洲2谱系），目前该地区尚未报告鸟类的重大死亡情况，并且在随机采集的黑鹂尸体中未发现黄病毒RNA。所获得的数据有助于我们了解USUV在中欧的遗传变异性、分布和传播情况。
