Changchun Sci-Tech University, 1699 DongHua St., Shuangyang District, Changchun, Jilin, 130022, China.
The Scientific Research Center, China-Japan Union Hospital of Jilin University, 126 Xiantai St., Changchun, Jilin, 130033, China.
Stem Cell Res Ther. 2019 Nov 19;10(1):326. doi: 10.1186/s13287-019-1457-9.
When the deer antler is cast, it leaves a cutaneous wound that can achieve scarless healing due to the presence of antler stem cells (ASCs). This provides an opportunity to study regenerative wound healing.
In this study, we investigated the therapeutic effects and mechanism of antler stem cell-conditioned medium (ASC-CM) on cutaneous wound healing in rats. In vitro, we investigated the effects of the ASC-CM on proliferation of HUVEC and NIH-3T3 cell lines. In vivo, we evaluated the effects of ASC-CM on cutaneous wound healing using full-thickness skin punch-cut wounds in rats.
The results showed that ASC-CM significantly stimulated proliferation of the HUVEC and NIH-3T3 cells in vitro. In vivo, completion of healing of the rat wounds treated with ASC-CM was on day 16 (± 3 days), 9 days (± 2 days) earlier than the control group (DMEM); the area of the wounds treated with ASC-CM was significantly smaller (p < 0.05) than the two control groups. Further molecular characterization showed that the ratios of Col3A1/Col1A2, TGF-β3/TGF-β1, MMP1/TIMP1, and MMP3/TIMP1 significantly increased (p < 0.01) in the healed tissue in the ASC-CM group.
In conclusion, ASC-CM effectively accelerated the wound closure rate and enhanced the quality of healing, which might be through transforming wound dermal fibroblasts into the fetal counterparts. Therefore, the ASC-CM may have potential to be developed as a novel cell-free therapeutic for scarless wound healing.
当鹿角被切除时，它会留下一个皮肤伤口，由于鹿角干细胞（ASCs）的存在，伤口可以实现无痕愈合。这为研究再生性伤口愈合提供了机会。
在这项研究中，我们研究了鹿角干细胞条件培养基（ASC-CM）对大鼠皮肤伤口愈合的治疗效果和机制。在体外，我们研究了 ASC-CM 对 HUVEC 和 NIH-3T3 细胞系增殖的影响。在体内，我们使用大鼠全层皮肤打孔伤评估了 ASC-CM 对皮肤伤口愈合的影响。
结果表明，ASC-CM 显著刺激了 HUVEC 和 NIH-3T3 细胞的体外增殖。在体内，用 ASC-CM 处理的大鼠伤口的愈合完成时间为第 16 天（±3 天），比对照组（DMEM）早 9 天（±2 天）；用 ASC-CM 处理的伤口面积明显小于两个对照组（p<0.05）。进一步的分子特征表明，在 ASC-CM 组愈合组织中，Col3A1/Col1A2、TGF-β3/TGF-β1、MMP1/TIMP1 和 MMP3/TIMP1 的比值显著增加（p<0.01）。
总之，ASC-CM 有效加速了伤口闭合率，并提高了愈合质量，这可能是通过将伤口真皮成纤维细胞转化为胎儿对应物实现的。因此，ASC-CM 可能有潜力被开发为一种无细胞的新型治疗方法，用于无痕愈合。
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