Unidad de Coordinación de Trasplantes, Terapia Celular y Medicina Regenerativa, Hospital Universitario Central de Asturias, Oviedo, Spain.
Departamento de Morfología y Biología Celular, Universidad de Oviedo, Oviedo, Spain.
Cell Mol Biol Lett. 2017 Dec 19;22:29. doi: 10.1186/s11658-017-0055-z. eCollection 2017.
Several recent studies have demonstrated the great potential of bone marrow cells in regenerative medicine, not only for their ability to differentiate to match a damaged cell type, but also because they synthesize and release various growth factors and cytokines.We examined the effect of bone marrow cell-conditioned medium in the healing process, especially in terms of fibroblast proliferation and migration.
These in vitro studies consisted of co-culture (without direct contact) of dermal fibroblasts with mononuclear bone marrow cells and the use of conditioned medium obtained from these cultures in a scratch wound model.
Mononuclear cells were found to increase the proliferation of fibroblasts, and the conditioned medium showed a stimulatory effect on the migration of fibroblasts.
When considered together with the observed increase in growth factor levels in conditioned medium, it appears that these cells act through a paracrine mechanism.
最近的几项研究表明，骨髓细胞在再生医学中有巨大的潜力，不仅因为它们能够分化以匹配受损的细胞类型，还因为它们能够合成和释放各种生长因子和细胞因子。我们研究了骨髓细胞条件培养基在愈合过程中的作用，特别是在成纤维细胞增殖和迁移方面的作用。
这些体外研究包括将真皮成纤维细胞与单核骨髓细胞共培养（无直接接触），并在划痕模型中使用从这些培养物中获得的条件培养基。
单核细胞被发现能增加成纤维细胞的增殖，而条件培养基对成纤维细胞的迁移有刺激作用。
结合观察到条件培养基中生长因子水平的升高，这些细胞似乎通过旁分泌机制发挥作用。
