骨髓单个核细胞条件培养液在人真皮成纤维细胞增殖和迁移中的作用。
The role of bone marrow mononuclear cell-conditioned medium in the proliferation and migration of human dermal fibroblasts.
机构信息
Unidad de Coordinación de Trasplantes, Terapia Celular y Medicina Regenerativa, Hospital Universitario Central de Asturias, Oviedo, Spain.
Departamento de Morfología y Biología Celular, Universidad de Oviedo, Oviedo, Spain.
出版信息
Cell Mol Biol Lett. 2017 Dec 19;22:29. doi: 10.1186/s11658-017-0055-z. eCollection 2017.
BACKGROUND
Several recent studies have demonstrated the great potential of bone marrow cells in regenerative medicine, not only for their ability to differentiate to match a damaged cell type, but also because they synthesize and release various growth factors and cytokines.We examined the effect of bone marrow cell-conditioned medium in the healing process, especially in terms of fibroblast proliferation and migration.
METHODS
These in vitro studies consisted of co-culture (without direct contact) of dermal fibroblasts with mononuclear bone marrow cells and the use of conditioned medium obtained from these cultures in a scratch wound model.
RESULTS
Mononuclear cells were found to increase the proliferation of fibroblasts, and the conditioned medium showed a stimulatory effect on the migration of fibroblasts.
CONCLUSION
When considered together with the observed increase in growth factor levels in conditioned medium, it appears that these cells act through a paracrine mechanism.
背景
最近的几项研究表明，骨髓细胞在再生医学中有巨大的潜力，不仅因为它们能够分化以匹配受损的细胞类型，还因为它们能够合成和释放各种生长因子和细胞因子。我们研究了骨髓细胞条件培养基在愈合过程中的作用，特别是在成纤维细胞增殖和迁移方面的作用。
方法
这些体外研究包括将真皮成纤维细胞与单核骨髓细胞共培养（无直接接触），并在划痕模型中使用从这些培养物中获得的条件培养基。
结果
单核细胞被发现能增加成纤维细胞的增殖，而条件培养基对成纤维细胞的迁移有刺激作用。
结论
结合观察到条件培养基中生长因子水平的升高，这些细胞似乎通过旁分泌机制发挥作用。
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