Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan.
Food Funct. 2019 Dec 11;10(12):7875-7882. doi: 10.1039/c9fo02050k.
Black soybean seed coat polyphenols were reported to possess various bioregulatory functions. However, the effects of black soybean seed coat polyphenols on vascular functions are unknown. Vascular dysfunction caused by aging and vascular stiffness is associated with a risk of cardiovascular disease (CVD), and a reduction in nitric oxide (NO) levels can trigger the onset of CVD. In the present study, we investigated the effect of polyphenol-rich black soybean seed coat extract (BE) on vascular functions and the underlying mechanisms involved. The oral administration of BE at 50 mg per kg body weight to Wistar rats increased NO levels as determined by eNOS phosphorylation. The administration of BE also increased GLP-1 and cAMP levels. Furthermore, the effects of BE were inhibited in the presence of a GLP-1 receptor antagonist. This suggests that GLP-1 is strongly involved in the underlying mechanism of NO production in vivo. In conclusion, BE contributes to the improvement of vascular functions by promoting NO production. Regarding the putative underlying mechanism, GLP-1 secreted from intestinal cells by the polyphenols in BE activates eNOS in vascular endothelial cells.
黑大豆种皮多酚被报道具有多种生物调节功能。然而，黑大豆种皮多酚对血管功能的影响尚不清楚。由衰老和血管僵硬引起的血管功能障碍与心血管疾病（CVD）的风险相关，一氧化氮（NO）水平的降低会引发 CVD 的发生。在本研究中，我们研究了富含多酚的黑大豆种皮提取物（BE）对血管功能的影响及其潜在的作用机制。通过测定 eNOS 磷酸化，发现给予 50mg/kg 体重的 BE 可增加 NO 水平。BE 的给药还增加了 GLP-1 和 cAMP 的水平。此外，在存在 GLP-1 受体拮抗剂的情况下，BE 的作用被抑制。这表明 GLP-1 强烈参与了体内 NO 产生的潜在机制。总之，BE 通过促进 NO 的产生来改善血管功能。关于潜在的作用机制，BE 中的多酚从肠细胞分泌 GLP-1，激活血管内皮细胞中的 eNOS。
