通过 HpaII 介导的连接 PCR 以单碱基分辨率高度灵敏地定量特异性 5-羟甲基胞嘧啶。
Highly sensitive quantification of site-specific 5-hydroxymethylcytosine at single-base resolution by HpaII-mediated ligation PCR.
机构信息
Key laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China.
School of Chemistry and Biology Engineering, University of Science and Technology Beijing, Beijing, 100083, P. R. China.
出版信息
Org Biomol Chem. 2019 Dec 14;17(46):9849-9853. doi: 10.1039/c9ob02429h. Epub 2019 Nov 20.
A highly sensitive HpaII-mediated ligation PCR assay that can precisely discriminate 5hmC from 5mC and C is developed for the quantitative determination of site-specific 5hmC in genomic DNA samples.
开发了一种高度敏感的 HpaII 介导的连接 PCR 检测法，该方法可精确区分 5hmC 与 5mC 和 C，用于定量测定基因组 DNA 样品中特定位点的 5hmC。
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