Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Oct 15;907:7-12. doi: 10.1016/j.jchromb.2012.08.012. Epub 2012 Aug 28.
Ginseng polysaccharide is known to have anti-hyperglycemic and anti-hyperlipidemic effects in vivo and its precise mechanism of action is not clear. A urinary metabolomics method based on rapid-resolution liquid chromatography/mass spectrometry (RRLC/MS) was developed to investigate the effect of water-soluble ginseng polysaccharide (WGP) on type 2 diabetes in rats. Principal component analysis (PCA) was carried out for pattern recognition and a clear separation between type 2 diabetic rats and those treated with WGP was achieved. Eight potential biomarkers were found and identified. Significantly increased inosine, serotonin, phenylpropionylglycine and dodecanedioic acid showed the effect of WGP on purine metabolism, tryptophan metabolism, fatty acid metabolism and energy metabolism. 1-Methyladenine, 4-deoxyerythronic acid, 5-hydroxyhexanoic acid and tetrahydrocortisol were significantly decreased which indicated that WGP can regulate DNA metabolism, organic acids metabolism and steroid hormone metabolism. This work is helpful in the effect mechanism study of ginseng polysaccharide.
人参多糖具有体内抗高血糖和抗高血脂作用，但确切的作用机制尚不清楚。本研究建立了一种基于快速分辨液相色谱/质谱（RRLC/MS）的尿液代谢组学方法，以研究水溶性人参多糖（WGP）对 2 型糖尿病大鼠的作用。采用主成分分析（PCA）进行模式识别，实现了 2 型糖尿病大鼠与 WGP 治疗大鼠的明显分离。发现并鉴定了 8 个潜在的生物标志物。肌苷、血清素、苯丙酰甘氨酸和十二烷二酸的显著增加表明 WGP 对嘌呤代谢、色氨酸代谢、脂肪酸代谢和能量代谢有影响。1-甲基腺嘌呤、4-脱氧赤藓糖酸、5-羟基己酸和四氢皮质醇显著降低，表明 WGP 可以调节 DNA 代谢、有机酸代谢和甾体激素代谢。这项工作有助于研究人参多糖的作用机制。
